Abstract

The specific aims are to analyze interactions among the embryonic neural tube and adjacent chordamesoderm during normal and abnormal development in mutant mice which exhibit neural dysraphism and in rhesus monkey embryos in which neural tube malformations have been induced by treatment with triamcinolone acetonide. A variety of techniques will be used at the electron microscopic level, including acid phosphatase cytochemistry, ruthenium red and tannic acid histochemistry, and alcian blue at critical electrolyte concentrations with differential enzyme digestion. Tritiated thymidine autoradiography at the light microscopic level will also be used. This project will help to contribute to an understanding of the etiology of specific human neurological disorders such as exencephaly, meningocele, anencephaly, and spina bifida.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS020055-02
Application #
3400256
Study Section
Neurology A Study Section (NEUA)
Project Start
1983-12-01
Project End
1986-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California San Diego
Department
Type
Schools of Medicine
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093

  Publications

Wilson, D B; Wyatt, D P (1995) Immunofluorescent analysis of the spinal cord in dysraphic mice. Acta Anat (Basel) 154:120-7
Wilson, D B; Wyatt, D P (1995) Alterations in cranial morphogenesis in the Lp mutant mouse. J Craniofac Genet Dev Biol 15:182-9
Wilson, D B; Wyatt, D P (1995) Patterns of lectin binding during mammalian neurogenesis. J Anat 186 ( Pt 1):209-16
Wilson, D B; Wyatt, D P (1994) Analysis of neurulation in a mouse model for neural dysraphism. Exp Neurol 127:154-8
Wilson, D B; Wyatt, D P (1993) In vitro expression of neural tube pathology in the vl mutant mouse. J Neuropathol Exp Neurol 52:253-9
Wilson, D B; Wyatt, D P (1992) Aberrant convergence of the neural folds in the mouse mutant vl. Teratology 45:105-12
Wilson, D B; Wyatt, D P (1992) Abnormal elevation of the neural folds in the loop-tail mutant mouse. Acta Anat (Basel) 143:89-95
Wilson, D B; Wyatt, D P (1991) Ultrastructural analysis of the midaxial extracellular matrix in spinal dysraphism. Virchows Arch B Cell Pathol Incl Mol Pathol 60:113-8
Wilson, D B; Wyatt, D P; Gookin, J L (1990) Cranial effects of retinoic acid in the loop-tail (Lp) mutant mouse. J Craniofac Genet Dev Biol 10:75-81
Wilson, D B; Wyatt, D P (1989) Axial interactions during neurogenesis in dysraphic mice. Anat Embryol (Berl) 180:515-9

Showing the most recent 10 out of 21 publications