This grant represents a revised proposal for the study of T cell clonality in multiple sclerosis cerebrospinal fluid by analysing rearrangement patterns of the T cell receptor genes. The studies outlined in the previous grant of peripheral blood and family genetic studies have been deleted from the proposal. New data since submission of the original proposal has been obtained demonstrating oligoclonal T cells in the spinal fluid of an MS patient. In addition, brain tissue has been obtained at an autopsy from a patient in whom T cells were cloned prior to the patient's death. This study will focus on oligoclonal T cells in the spinal fluid of multiple sclerosis patients according to the following specific aims: 1) to what extent are cerebrospinal fluid, oligoclonal T cell populations found in patients with chronic progressive multiple sclerosis? 2) are oligoclonal T cell populations found in early relapsing-remitting patients? 3) do CSF T cell clones reflect the population of T cells in the plaque of MS brain? and 4) what are the functional properties of oligoclonal T cell populations?

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS024247-02
Application #
3408606
Study Section
Immunological Sciences Study Section (IMS)
Project Start
1987-07-01
Project End
1990-06-30
Budget Start
1988-07-01
Budget End
1989-06-30
Support Year
2
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Brigham and Women's Hospital
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02115
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