Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS033650-03
Application #
2272569
Study Section
Neurological Sciences Subcommittee 1 (NLS)
Project Start
1994-09-30
Project End
1997-09-29
Budget Start
1996-09-30
Budget End
1997-09-29
Support Year
3
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Pharmacology
Type
Schools of Medicine
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Gopalakrishnan, Anupama; Sievert, Michael; Ruoho, Arnold E (2007) Identification of the substrate binding region of vesicular monoamine transporter-2 (VMAT-2) using iodoaminoflisopolol as a novel photoprobe. Mol Pharmacol 72:1567-75
Sievert, Michael K; Hajipour, Abdol R; Ruoho, Arnold E (2007) Specific derivatization of the vesicle monoamine transporter with novel carrier-free radioiodinated reserpine and tetrabenazine photoaffinity labels. Anal Biochem 367:68-78
Thiriot, David S; Sievert, Michael K; Ruoho, Arnold E (2002) Identification of human vesicle monoamine transporter (VMAT2) lumenal cysteines that form an intramolecular disulfide bond. Biochemistry 41:6346-53
Thiriot, D S; Ruoho, A E (2001) Mutagenesis and derivatization of human vesicle monoamine transporter 2 (VMAT2) cysteines identifies transporter domains involved in tetrabenazine binding and substrate transport. J Biol Chem 276:27304-15
Cozzi, N V; Sievert, M K; Shulgin, A T et al. (1999) Inhibition of plasma membrane monoamine transporters by beta-ketoamphetamines. Eur J Pharmacol 381:63-9
Sievert, M K; Thiriot, D S; Edwards, R H et al. (1998) High-efficiency expression and characterization of the synaptic-vesicle monoamine transporter from baculovirus-infected insect cells. Biochem J 330 ( Pt 2):959-66
Sievert, M K; Ruoho, A E (1997) Peptide mapping of the [125I]Iodoazidoketanserin and [125I]2-N-[(3'-iodo-4'-azidophenyl)propionyl]tetrabenazine binding sites for the synaptic vesicle monoamine transporter. J Biol Chem 272:26049-55