This proposal focuses on the development of somatosensory thalamocortical circuitry in mice with genetically impaired NMDAR function. Rodent somatosensory pathway is an excellent model system to study development of topographic connections and patterning within somatosensory maps. Somatosensory patterns are abolished in the brainstem of mice lacking the critical subunit of the NMDARs. Mice that express lower levels of NMDAR function also show absence of patterning all along the somatosensory pathway. Mice with cortex-restricted disruption of NMDARs in excitatory neurons also display severe defects in cortical patterning within the somatosensory body map region. Thus, somatosensory region-specific knockout mouse models provide an excellent means to dissect out the role of NMDARs and downstream signaling molecules in patterning of pre- and postsynaptic neural elements. The long-term objective of this proposal is to reveal how somatosensory synaptic circuitry is altered following impaired NMDAR function. In vivo and in vitro electrophysiological approaches will be used to elucidate functional changes in the somatosensory cortex and thalamus of these mice. These studies will complement ongoing morphological analyses and overall reveal the role of NMDARs in organization and patterning of somatosensory neural maps.

Public Health Relevance

NMDA receptors (NMDARs) play a major role in brain development, learning and memory, developmental neurological disorders, addiction, pain perception and in excitotoxicity. In this proposal we shall test the role of NMDARs in normal development of somatosensory neural circuits by examining physiological defects in region-specific genetic invalidation of NMDARs in mouse models. Understanding basic cellular and molecular mechanisms of NMDAR function in normal brain development is crucial for critical evaluation of developmental brain disorders and in developing therapeutic strategies.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Research Project (R01)
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Somatosensory and Chemosensory Systems Study Section (SCS)
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Riddle, Robert D
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University of Maryland Baltimore
Anatomy/Cell Biology
Schools of Medicine
United States
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Tang, Qinggong; Lin, Jonathan; Tsytsarev, Vassiliy et al. (2017) Review of mesoscopic optical tomography for depth-resolved imaging of hemodynamic changes and neural activities. Neurophotonics 4:011009
Lo, Fu-Sun; Erzurumlu, Reha S (2016) Neonatal sensory nerve injury-induced synaptic plasticity in the trigeminal principal sensory nucleus. Exp Neurol 275 Pt 2:245-52
Tang, Qinggong; Tsytsarev, Vassiliy; Frank, Aaron et al. (2016) In Vivo Mesoscopic Voltage-Sensitive Dye Imaging of Brain Activation. Sci Rep 6:25269
Tsytsarev, Vassiliy; Pumbo, Elena; Tang, Qinggong et al. (2016) Study of the cortical representation of whisker frequency selectivity using voltage-sensitive dye optical imaging. Intravital 5:e1142637
Arakawa, Hiroyuki; Erzurumlu, Reha S (2015) Role of whiskers in sensorimotor development of C57BL/6 mice. Behav Brain Res 287:146-55
Suzuki, A; Lee, L-J; Hayashi, Y et al. (2015) Thalamic adenylyl cyclase 1 is required for barrel formation in the somatosensory cortex. Neuroscience 290:518-29
Tang, Qinggong; Tsytsarev, Vassiliy; Liang, Chia-Pin et al. (2015) In Vivo Voltage-Sensitive Dye Imaging of Subcortical Brain Function. Sci Rep 5:17325
Arakawa, Hiroyuki; Akkentli, Fatih; Erzurumlu, Reha S (2014) Region-Specific Disruption of Adenylate Cyclase Type 1 Gene Differentially Affects Somatosensorimotor Behaviors in Mice. eNeuro 1:
Arakawa, Hiroyuki; Akkentli, Fatih; Erzurumlu, Reha S (2014) Region-Specific Disruption of Adenylate Cyclase Type 1 Gene Differentially Affects Somatosensorimotor Behaviors in Mice(1,2,3). eNeuro 1:
Arakawa, Hiroyuki; Suzuki, Ayumi; Zhao, Shuxin et al. (2014) Thalamic NMDA receptor function is necessary for patterning of the thalamocortical somatosensory map and for sensorimotor behaviors. J Neurosci 34:12001-14

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