Alzheimer's disease (AD) is the most common neurodegenerative disorder characterized by dementia and neurodegeneration. Mutations in the Presenilin (PSEN) genes account for >80% of all causative mutations identified in familial AD, highlighting its importance in the pathogenesis of AD. Through the development and multidisciplinary analysis of a series of Psen mutant mice, we previously established that Presenilin (PS) is essential for learning and memory, synaptic function and neuronal survival. For example, presynaptic PS plays a selective role in neurotransmitter release and synaptic plasticity through the regulation of ryanodine receptors (RyRs) and RyR-mediated calcium release from the ER. Synaptic dysfunction has widely been considered to play a key role in AD pathogenesis, and the hippocampal network is particularly vulnerable in AD. However, published reports have largely focused on the hippocampal Schaffer collateral pathway, and rarely explored in the mossy fiber and the perforant path. Furthermore, inhibitory interneurons play an integral role in the regulation of neuronal networks, but it is entirely unknown whether PS plays a normal physiological role in interneurons. In this competing renewal application, we propose two Specific Aims to investigate the role of PS in the regulation of the hippocampal network and the pathogenic mechanism of familial AD.
In Aim 1, we will determine and compare the role of PS in the tri-synaptic loop of the hippocampus. Specifically, we will determine the consequences of PS inactivation in mossy fiber and perforant synapses, relative to Schaffer collateral synapses, using acute hippocampal slices. We will also investigate the role of PS in inhibitory interneurons through the generation and analysis of interneuron-specific conditional double knockout mice.
In Aim 2, we will investigate the role of PS in familial AD. Specifically, we will compare mouse neurons derived from knockin mice carrying the pathogenic PSEN1 L435F or C410Y mutation with human neurons derived from iPS cells carrying the same mutation, and will assess the effects of the mutation on ?-secretase activity, calcium regulation and synaptic function between the mouse and human neurons. The completion of the proposed study will provide mechanistic insight into PS function and dysfunction in excitatory and inhibitory neurons as well as its involvement in familial AD.

Public Health Relevance

Alzheimer's disease (AD) is the most common neurodegenerative disorder, and mutations in the Presenilin genes are the most common cause of the inherited forms of the disease. The hippocampus is particularly vulnerable in AD, and synaptic dysfunction is widely considered as the earliest pathogenic event. In this application, we propose to study the normal function of Presenilins in the hippocampal network and how the mutations cause familial AD. Completion of our proposed study will provide insights into AD pathogenesis and may identify novel therapeutic targets for AD.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS041783-18
Application #
9626965
Study Section
Cell Death in Neurodegeneration Study Section (CDIN)
Program Officer
Corriveau, Roderick A
Project Start
2001-05-15
Project End
2021-01-31
Budget Start
2019-02-01
Budget End
2020-01-31
Support Year
18
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Brigham and Women's Hospital
Department
Type
DUNS #
030811269
City
Boston
State
MA
Country
United States
Zip Code
02115
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