The goal of this research project is to define the logic by which the brain organizes different types of memories among its component neurons. The project contrasts how the brain organizes olfactory memories learned in association with a rewarding cue and those learned in association with an aversive cue, and delves into some of the underlying mechanisms. The research project utilizes the model system Drosophila melanogaster because of the ease in conditioning the fly using olfactory cues and because of the ability to peer into the brain of living animals and watch the activity of different sets of neurons. The latter approach, functional optical cellular imaging, employs flies carrying transgenes expressing reporters for calcium influx, synaptic transmission, or other neuronal events, to monitor changes in neuronal response properties among the expressing neurons before and after conditioning. To date, six different cellular memory traces have been defined using aversive olfactory conditioning. These traces form in different sets of neurons in the olfactory nervous system and occur with differing temporal dynamics. The memory traces that occur within these same neurons after a rewarding olfactory conditioning event will be examined along with the molecular mechanisms underlying memory trace formation. Since nearly every neuropsychiatric disorder affects memory formation, these studies will aid in understanding memory formation in the normal brain as well as in the diseased brain.

Public Health Relevance

The majority of human neurological and psychiatric disorders involve impairment in learning and memory. This project will examine the logic of how the brain encodes different types of memories, contrasting memories associated with rewarding cues versus memories associated with aversive cues, along with detailing the underlying molecular mechanisms. We will utilize the model Drosophila melanogaster - because of the ease of studying memory formation in this organism and the ability to peer into its brain and watch the activity of neurons during learning - to obtain knowledge that will contribute to understanding how the brain, including the human brain, organizes different types of memories and how brain disorders disrupt learning and memory.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS052351-07
Application #
8551735
Study Section
Neurobiology of Learning and Memory Study Section (LAM)
Program Officer
Mamounas, Laura
Project Start
2005-07-01
Project End
2017-08-31
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
7
Fiscal Year
2013
Total Cost
$475,477
Indirect Cost
$235,337
Name
Scripps Florida
Department
Type
DUNS #
148230662
City
Jupiter
State
FL
Country
United States
Zip Code
33458
Berry, Jacob A; Davis, Ronald L (2014) Active forgetting of olfactory memories in Drosophila. Prog Brain Res 208:39-62
Guven-Ozkan, Tugba; Davis, Ronald L (2014) Functional neuroanatomy of Drosophila olfactory memory formation. Learn Mem 21:519-26
Tan, Ying; Yu, Dinghui; Busto, Germain U et al. (2013) Wnt signaling is required for long-term memory formation. Cell Rep 4:1082-9
Cervantes-Sandoval, Isaac; Martin-Pena, Alfonso; Berry, Jacob A et al. (2013) System-like consolidation of olfactory memories in Drosophila. J Neurosci 33:9846-54
Akalal, David-Benjamin G; Yu, Dinghui; Davis, Ronald L (2011) The long-term memory trace formed in the Drosophila ýý/ýý mushroom body neurons is abolished in long-term memory mutants. J Neurosci 31:5643-7
Davis, Ronald L (2011) Traces of Drosophila memory. Neuron 70:8-19
Liu, Xu; Buchanan, Monica E; Han, Kyung-An et al. (2009) The GABAA receptor RDL suppresses the conditioned stimulus pathway for olfactory learning. J Neurosci 29:1573-9
Liu, Xu; Davis, Ronald L (2009) The GABAergic anterior paired lateral neuron suppresses and is suppressed by olfactory learning. Nat Neurosci 12:53-9
Tomchik, Seth M; Davis, Ronald L (2009) Dynamics of learning-related cAMP signaling and stimulus integration in the Drosophila olfactory pathway. Neuron 64:510-21
Liu, Xu; Krause, William C; Davis, Ronald L (2007) GABAA receptor RDL inhibits Drosophila olfactory associative learning. Neuron 56:1090-102

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