Experimental autoimmune encephalomyelitis (EAE) serves as a model for human multiple sclerosis (MS). Both EAE and MS manifest as a consequence of an inflammatory process within the central nervous system (CNS) that is mediated by cells of the immune system. The disease develops when T regulatory cells (Tregs) are no longer able to control activation of myelin-reactive effector T cells. It is thus crucial to understand why Tregs become unable to keep effector T cells in check and how the myelin-reactive lymphocytes evade such control in order to develop approaches that could contain the disease. Recently, we discovered a novel class of thymic stem cell progenitors expressing IL-13 receptor ?1 (IL-13R?1) that contribute to the development and function of both Tregs and effector T cells. The lineage negative (Lin-) IL-13R?1+ stem cells, which arise in the bone marrow (BM), migrate to the thymus and become early thymic progenitors (ETPs). However, these IL-13R?1+ ETPs do not mature into T lymphocytes. Rather, they give rise to CD11b+ myeloid cells that are retained in the thymus and function as antigen-presenting cells (APCs) that contribute to the selection process of both Tregs and effector T cells. The IL-13R?1+ BM stem cells that do not migrate to the thymus also give rise to IL-13R?1+ myeloid cells as well as dendritic cells (DCs) that likely function as peripheral APCs and contribute to the function of Tregs and effector T cells. The long term objective in this proposal is to define the contribution of IL-13R?1+ ETPs to thymic T cell selection and to determine how the IL-13R?1+ BM stem cell-derived peripheral APCs (myeloid and DCs) regulate the function of the ETP-selected Tregs and effector T cells. The major hypothesis in this proposal postulates that IL-13R?1+ETP-derived myeloid cells function as APCs for thymic selection and display discrepancies among effector and regulatory T cells as well as self versus foreign antigens. Ultimately, the thymic outcome dictates T cell output to the periphery and, in coordination with the IL- 13R?1+ peripheral APCs, regulates myelin-reactivity and EAE.
Three aims are proposed to test these hypotheses.
Aim 1 will define the role IL-13R?1+ETPs play in thymic T cell selection, aim 2 will assess the role IL-13R?1+ ETPs play in the development of peripheral T cell responses and EAE, and aim 3 will determine how IL-13R?1+ Lin- BM stem cells regulate T cell responses and EAE. This study is of high impact because it could yield mechanistic insights useful for the generation of therapeutics against EAE and MS.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS057194-06
Application #
8656155
Study Section
Clinical Neuroimmunology and Brain Tumors Study Section (CNBT)
Program Officer
Utz, Ursula
Project Start
2007-01-01
Project End
2018-03-31
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
6
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Missouri-Columbia
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
City
Columbia
State
MO
Country
United States
Zip Code
65211
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Barik, Subhasis; Miller, Mindy; Cattin-Roy, Alexis et al. (2018) A distinct dendritic cell population arises in the thymus of IL-13R?1-sufficient but not IL-13R?1-deficient mice. Cell Immunol 331:130-136
Barik, Subhasis; Ellis, Jason S; Cascio, Jason A et al. (2017) IL-4/IL-13 Heteroreceptor Influences Th17 Cell Conversion and Sensitivity to Regulatory T Cell Suppression To Restrain Experimental Allergic Encephalomyelitis. J Immunol 199:2236-2248
Barik, Subhasis; Miller, Mindy M; Cattin-Roy, Alexis N et al. (2017) IL-4/IL-13 Signaling Inhibits the Potential of Early Thymic Progenitors To Commit to the T Cell Lineage. J Immunol 199:2767-2776
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Cascio, Jason A; Haymaker, Cara L; Divekar, Rohit D et al. (2013) Antigen-specific effector CD4 T lymphocytes school lamina propria dendritic cells to transfer innate tolerance. J Immunol 190:6004-14
Wan, Xiaoxiao; Guloglu, F Betul; VanMorlan, Amie M et al. (2012) Mechanisms underlying antigen-specific tolerance of stable and convertible Th17 cells during suppression of autoimmune diabetes. Diabetes 61:2054-65
Haymaker, Cara L; Guloglu, F Betul; Cascio, Jason A et al. (2012) Bone marrow-derived IL-13Rýý1-positive thymic progenitors are restricted to the myeloid lineage. J Immunol 188:3208-16

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