Despite aggressive therapies surgical resection, radiation, and chemotherapy, Glioblastoma Multiforme (GBM), the most common primary malignant brain tumor, remains uniformly lethal due to recurrence and therapeutic resistance. The inability to adequately treat these tumors may be due in part to a subset of tumor cells, cancer stem cells, that are resistant to many conventional therapies. Cancer stem cells within GBMs are localized to several areas, among them the perivascular compartment, which is a known cancer stem cell microenvironment or niche and has been shown to play a role in therapeutic resistance. Adhesion is a hallmark of the stem cell state and identifying adhesion mechanisms specifically used by CSC to communicate with their microenvironment has is of immediate importance and implications in the design of more effective glioma therapies. We have recently uncovered a CSC-specific adhesion mechanism, junctional adhesion molecule-A (JAM-A), and our preliminary data show that JAM-A can be targeted to attenuate CSC phenotypes but has no major impact on normal neural function. Based on these findings, the hypothesis of this proposal is JAM-A specifically regulates niche interactions in CSC and is a potent anti-GBM target. This hypothesis will be evaluated by: 1) interrogating the regulatory axis of JAM-A to show the molecular mechanism of JAM-A regulation;2) determining how JAM-A targeting inhibits CSC function but not normal neural cell function;and 3) evaluating how targeting JAM-A in combination with radiation and chemotherapy impacts CSC-driven tumor growth. Experimental studies will utilize human GBM specimens and normal neural progenitor cells to evaluate JAM-A targeting by RNA interference or blocking antibody administration. Function will be assessed using a variety of CSC assays and will include evaluation using an intravital imaging model to study the in vivo communication between CSC and the niche in the context of JAM-A inhibition. The long term objective of this proposal is to develop GBM therapies with increased therapeutic efficacy that target the CSC in combination with conventional therapies, while not compromising normal neural function. These studies outlined in this proposal will uncover the critical role of CSC interaction with the niche via JAM-A and evaluate potential therapies to GBM which disrupt niche related communication. Any findings and therapeutic developments may extend to other tumor types with a cancer stem cell component (i.e. colon, breast).
Glioblastoma Multiforme (GBM) is the most common malignant primary brain tumor and among the most lethal due to recurrence and therapeutic resistance. These properties that are associated with a cancer stem cell (CSC) population and the research proposed in this application aims to understand how GBM cell interaction with the surrounding microenvironment via novel adhesion mechanisms. The application will interrogate the role of junctional adhesion molecule-A (JAM-A), a newly defined CSC-specific adhesion mechanism, in promoting CSC phenotypes. The successful completion of this proposal will demonstrate the effectiveness of targeting JAM-A in CSCs with limited toxicity to normal neural cells, thereby facilitating its testing as an anti- GBM therapy in a clinical setting.
|Walters, M J; Ebsworth, K; Berahovich, R D et al. (2014) Inhibition of CXCR7 extends survival following irradiation of brain tumours in mice and rats. Br J Cancer 110:1179-88|
|Lathia, Justin D; Li, Meizhang; Sinyuk, Maksim et al. (2014) High-throughput flow cytometry screening reveals a role for junctional adhesion molecule a as a cancer stem cell maintenance factor. Cell Rep 6:117-29|
|Liu, J K; Lubelski, D; Schonberg, D L et al. (2014) Phage display discovery of novel molecular targets in glioblastoma-initiating cells. Cell Death Differ 21:1325-39|
|Hale, James S; Otvos, Balint; Sinyuk, Maksim et al. (2014) Cancer stem cell-specific scavenger receptor 36 drives glioblastoma progression. Stem Cells 32:1746-58|
|Hale, James S; Sinyuk, Maksim; Rich, Jeremy N et al. (2013) Decoding the cancer stem cell hypothesis in glioblastoma. CNS Oncol 2:319-30|