Voltage-gated Cav channels mediate activity-dependent Ca2+ signals required for gene transcription and neurotransmission. Ca2+-dependent inactivation and facilitation (CDI and CDF, respectively) allow Cav channels to adjust Ca2+ influx according to neuronal activity, thereby fine-tuning Ca2+ signals that control neuronal excitability and synaptic plasticity. The rationale for the proposed research is that defining how Cav channels generate and maintain Ca2+ signals will answer longstanding questions regarding the heterogeneous properties of Cav channels in neurons and enable new mechanistic inquiries into the roles of specific Cav channels in orchestrating the normal development and function of the nervous system. The expected outcomes of the proposed research are: establishment of a new role for calretinin as a dynamic regulator of effectors including Cav2.
1 (Aim 1); and elucidation of a mechanism responsible for the long-lasting properties and functional impact of neuronal Cav1 L-type currents (Aim 2). We believe that the proposed research will make a lasting and positive impact: the Cav channel regulatory mechanisms it will define will likely facilitate the development of novel therapeutics for neurological and neuropsychiatric disorders resulting from dysregulation of neuronal Ca2+ signals.

Public Health Relevance

The proposed research will characterize the mechanisms and physiological significance of factors that modulate voltage-gated Ca2+ channels neurons. We will elucidate new cellular and molecular mechanisms, which may be altered in neurological and neuropsychiatric diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS084190-04
Application #
9179667
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Silberberg, Shai D
Project Start
2013-12-15
Project End
2018-11-30
Budget Start
2016-12-01
Budget End
2017-11-30
Support Year
4
Fiscal Year
2017
Total Cost
Indirect Cost
Name
University of Iowa
Department
Physiology
Type
Schools of Medicine
DUNS #
062761671
City
Iowa City
State
IA
Country
United States
Zip Code
52242
Kerov, Vasily; Laird, Joseph G; Joiner, Mei-Ling et al. (2018) ?2?-4 Is Required for the Molecular and Structural Organization of Rod and Cone Photoreceptor Synapses. J Neurosci 38:6145-6160
Williams, Brittany; Haeseleer, Françoise; Lee, Amy (2018) Splicing of an automodulatory domain in Cav1.4 Ca2+ channels confers distinct regulation by calmodulin. J Gen Physiol 150:1676-1687
Yang, Tian; Choi, Ji-Eun; Soh, Daniel et al. (2018) CaBP1 regulates Cav1 L-type Ca2+ channels and their coupling to neurite growth and gene transcription in mouse spiral ganglion neurons. Mol Cell Neurosci 88:342-352
Thomas, Jessica R; Hagen, Jussara; Soh, Daniel et al. (2018) Molecular moieties masking Ca2+-dependent facilitation of voltage-gated Cav2.2 Ca2+ channels. J Gen Physiol 150:83-94
Garza-Lopez, Edgar; Lopez, Josue A; Hagen, Jussara et al. (2018) Role of a conserved glutamine in the function of voltage-gated Ca2+ channels revealed by a mutation in human CACNA1D. J Biol Chem 293:14444-14454
Nanou, Evanthia; Lee, Amy; Catterall, William A (2018) Control of Excitation/Inhibition Balance in a Hippocampal Circuit by Calcium Sensor Protein Regulation of Presynaptic Calcium Channels. J Neurosci 38:4430-4440
Yang, Tian; Britt, Jeremiah K; Cintrón-Pérez, Coral J et al. (2018) Ca2+-Binding Protein 1 Regulates Hippocampal-dependent Memory and Synaptic Plasticity. Neuroscience 380:90-102
Krueger, Jamie N; Moore, Shannon J; Parent, Rachel et al. (2017) A novel mouse model of the aged brain: Over-expression of the L-type voltage-gated calcium channel CaV1.3. Behav Brain Res 322:241-249
Wang, Xiaohan; Marks, Christian R; Perfitt, Tyler L et al. (2017) A novel mechanism for Ca2+/calmodulin-dependent protein kinase II targeting to L-type Ca2+ channels that initiates long-range signaling to the nucleus. J Biol Chem 292:17324-17336
Martínez-Rivera, A; Hao, J; Tropea, T F et al. (2017) Enhancing VTA Cav1.3 L-type Ca2+ channel activity promotes cocaine and mood-related behaviors via overlapping AMPA receptor mechanisms in the nucleus accumbens. Mol Psychiatry 22:1735-1745

Showing the most recent 10 out of 28 publications