Topographic representation of the sensory space in the brain is essential for sensory information processing and perception. The somatosensory and motor cortical maps in each hemisphere represent the contralateral body and the face. This is due to midline crossing of the ascending (sensory) and descending (motor) pathways at the level of the medulla or the pons. Genetic and developmental defects in midline crossing or injury at the crossing site severely affect sensory-motor information processing and actions in both animals and humans. In this proposal we use a region-specific gene deletion mouse model to study the consequences of partial crossing of the ascending somatosensory face pathway. Midline crossing defects in this mouse leads to bilateral face representation in the thalamus and subsequently in the somatosensory cortex. We will use this mouse model to investigate (a) morphological and electrophysiological properties of the pre and postsynaptic elements in the """"""""bifacial"""""""" cortical map;(b) altered thalamocortical and corticocortical connectivity patterns in response to bilateral face representation;(c) behavioral consequences of this genetic mutation. A combination of molecular, morphological, electrophysiological, voltage-sensitive dye imaging and behavioral techniques will be used to elucidate mechanisms underlying the functional organization and behavioral manifestations of developmental injury-related or genetic defects in ascending somatosensory pathways.
Perception of body and face sensations occurs through information processing in neural maps formed by somatosensory pathways of the brain. In particular, the neocortex has a disproportionate map of the face and body, which reflects the density of sensory receptors in the periphery. Sensory maps of each brain hemisphere process information from the opposite side of the body. Developmental injury or congenital defects in somatosensory pathways result in abnormal map formation. We will investigate the functional and behavioral consequences of such a map defect in a transgenic mouse model with duplicated face maps in each brain hemisphere.
|Kwon, Sung Eun; Tsytsarev, Vassiliy; Erzurumlu, Reha S et al. (2018) Organization of orientation-specific whisker deflection responses in layer 2/3 of mouse somatosensory cortex. Neuroscience 368:46-56|
|Mueller, Bernhard J; Zhdanov, Alexander V; Borisov, Sergey M et al. (2018) Nanoparticle-based fluoroionophore for analysis of potassium ion dynamics in 3D tissue models and in vivo. Adv Funct Mater 28:|
|Iwasato, Takuji; Erzurumlu, Reha S (2018) Development of tactile sensory circuits in the CNS. Curr Opin Neurobiol 53:66-75|
|Renier, Nicolas; Dominici, Chloé; Erzurumlu, Reha S et al. (2017) A mutant with bilateral whisker to barrel inputs unveils somatosensory mapping rules in the cerebral cortex. Elife 6:|
|Tsytsarev, Vassiliy; Arakawa, Hiroyuki; Zhao, Shuxin et al. (2017) Behavioral Consequences of a Bifacial Map in the Mouse Somatosensory Cortex. J Neurosci 37:7209-7218|
|Tang, Qinggong; Lin, Jonathan; Tsytsarev, Vassiliy et al. (2017) Review of mesoscopic optical tomography for depth-resolved imaging of hemodynamic changes and neural activities. Neurophotonics 4:011009|
|Tsytsarev, Vassiliy; Akkentli, Fatih; Pumbo, Elena et al. (2017) Planar implantable sensor for in vivo measurement of cellular oxygen metabolism in brain tissue. J Neurosci Methods 281:1-6|
|Nag, Okhil K; Stewart, Michael H; Deschamps, Jeffrey R et al. (2017) Quantum Dot-Peptide-Fullerene Bioconjugates for Visualization of in Vitro and in Vivo Cellular Membrane Potential. ACS Nano 11:5598-5613|
|Tang, Qinggong; Tsytsarev, Vassiliy; Frank, Aaron et al. (2016) In Vivo Mesoscopic Voltage-Sensitive Dye Imaging of Brain Activation. Sci Rep 6:25269|
|Tsytsarev, Vassiliy; Pumbo, Elena; Tang, Qinggong et al. (2016) Study of the cortical representation of whisker frequency selectivity using voltage-sensitive dye optical imaging. Intravital 5:e1142637|
Showing the most recent 10 out of 17 publications