The 'Layering Hypothesis,'first proposed in by Herzenberg in 1989, theorized that there are lineages of lymphocytes arising at different stages of development. A basal stratum of so-called "innate lymphocytes," the B1 cells and ?? T cells, was proposed to provide a first line of immune defense as well as other functions such as maintenance of tissue homeostasis. Recent data from mouse and human strongly support the 'layering hypothesis,'but little is known of such lineages over evolutionary time. The immunoglobulin (Ig) gene organization in cartilaginous fish (sharks, skates, and rays), the so-called cluster organization, allows for plasticity in the types of genes that can be selected and used in different ways;for example, some Ig heavy chain clusters have been selected for adaptive immunity with single-domain variable regions and others are 'germline-joined,'meaning that the rearranging gene segments were joined by RAG in germ cells and perpetuated in the population as fixed genes. One particular germline-joined heavy chain makes up over half of the serum Ig in neonates, and consistent with what has been shown in mice, the early antibody recognizes apoptotic cells, suggesting that it is involved in homeostatic functions. We will continue to study the structure and function of this molecule, concentrating on its ligand specificity. This IgM is expressed in plasma cells as a first wave during development, followed by at least two other waves of plasma cells. The 2nd wave expresses exclusively a multimeric form of IgM (19S) and is identified as J chain- positive/BLIMP1-negative, and the 3rd wave is J chain-negative/BLIMP1-positive (7S). The dichotomy in BLIMP1 and J chain expression suggests that it might be used as a universal marker for plasma cell lineages, and we plan to test this proposal in mouse and Xenopus, representatives of two other highly divergent vertebrate taxa. We will also examine the structure and function of ?? T cell receptors in Xenopus and sharks, two species in which immunoglobulin variable regions are used in a large proportion of the ? chains. Based on this finding, as well as uncovering such Ig/TCR chimeras in many other vertebrates, suggests that there are subpopulations of "adaptive ?? T cell receptors" in all vertebrates, which has not been appreciated. The study of this system will not only uncover layers of ?? T cell and B cell development in the oldest animals with adaptive immunity based on Ig/TCR/MHC, but we hypothesize that it may serve as a simple paradigm for layering of lymphocyte lineages in all other vertebrates.

Public Health Relevance

Study of the evolution of the immune system permits an outlook of those characteristics of immunity that are vital for defense in all animals versus those that are primitive or specific to particular species. Our research in comparative immunology gives an overall view of immune molecules such as antibodies that fight infectious diseases and cancer and avoid autoimmunity. In early life, we seem to make antibodies of a primitive nature that are important for clearing debris from the body;sharks have specialized in producing such antibodies in a unique way that we plan to study in this proposal.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Research Project (R01)
Project #
2R01OD010549-21A1
Application #
8578698
Study Section
Cellular and Molecular Immunology - B Study Section (CMIB)
Program Officer
Moro, Manuel H
Project Start
2013-07-15
Project End
2018-04-30
Budget Start
2013-07-15
Budget End
2014-04-30
Support Year
21
Fiscal Year
2013
Total Cost
$319,653
Indirect Cost
$111,410
Name
University of Maryland Baltimore
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
188435911
City
Baltimore
State
MD
Country
United States
Zip Code
21201
Castro, Caitlin D; Flajnik, Martin F (2014) Putting J chain back on the map: how might its expression define plasma cell development? J Immunol 193:3248-55
Feige, Matthias J; Gräwert, Melissa A; Marcinowski, Moritz et al. (2014) The structural analysis of shark IgNAR antibodies reveals evolutionary principles of immunoglobulins. Proc Natl Acad Sci U S A 111:8155-60
Venkatesh, Byrappa; Lee, Alison P; Ravi, Vydianathan et al. (2014) Elephant shark genome provides unique insights into gnathostome evolution. Nature 505:174-9