Dogs play a central role in laboratory medicine, ranging from studies of toxicology and pharmacokinetics to genetic therapy and bone marrow transplantation. Emerging papillomavirus infections and the tumors that they induce are increasingly complicating the study of immunosuppressed dogs. Defective immune responses in dogs are primarily the consequence of studying new drugs for treating dermatologic, neurologic and immunologic diseases. In addition, pioneering experiments on bone marrow transplantation also interfere with normal host immunity and make dogs susceptible to papillomavirus infection. To address this issue, we propose to identify the canine papillomaviruses responsible for these infections, to evaluate the time at which dogs are infected, and to develop prophylactic vaccines. The ultimate goal of our studies is to develop vaccines that will allow investigators to carry out medical research without being compromised by serious papillomavirus infections.
Dogs provide important laboratory models for the study of human disease and for the evaluation of drug safety. Many of these scientific investigations involve conditions that result in reduced host immunity. Under such conditions, dogs often develop skin tumors that interfere with the completion of the experimental protocol. The goal of this grant is to define whether papillomaviruses are responsible for most of these skin tumors and, if they are, what types of papillomaviruses are involved. Finally, the most important goal is to develop vaccines that can be used to protect the dogs against this viral infection and to prevent the development of tumors.
|Zhou, Dan; Luff, Jennifer; Usuda, Yukari et al. (2014) Complete genome sequence of canine papillomavirus type 11. Genome Announc 2:|
|Palechor-Ceron, Nancy; Suprynowicz, Frank A; Upadhyay, Geeta et al. (2013) Radiation induces diffusible feeder cell factor(s) that cooperate with ROCK inhibitor to conditionally reprogram and immortalize epithelial cells. Am J Pathol 183:1862-70|
|Luff, Jennifer A; Yuan, Hang; Suter, Maja M et al. (2013) Canine keratinocytes upregulate type I interferons and proinflammatory cytokines in response to poly(dA:dT) but not to canine papillomavirus. Vet Immunol Immunopathol 153:177-86|
|Yuan, Hang; Zhou, Dan; Wang, Jingang et al. (2013) Divergent human papillomavirus associated with recurrent respiratory papillomatosis with lung involvement. Genome Announc 1:|
|Luff, Jennifer; Moore, Peter; Zhou, Dan et al. (2012) Complete genome sequence of canine papillomavirus type 10. J Virol 86:11407|
|Yuan, Hang; Myers, Scott; Wang, Jingang et al. (2012) Use of reprogrammed cells to identify therapy for respiratory papillomatosis. N Engl J Med 367:1220-7|
|Yuan, Hang; Luff, Jennifer; Zhou, Dan et al. (2012) Complete genome sequence of canine papillomavirus type 9. J Virol 86:5966|
|Suprynowicz, Frank A; Upadhyay, Geeta; Krawczyk, Ewa et al. (2012) Conditionally reprogrammed cells represent a stem-like state of adult epithelial cells. Proc Natl Acad Sci U S A 109:20035-40|