In this research, responding to PA-13-129 Occupational Safety and Health Research (R01), an advanced bioaerosol sampler prototype with very high concentration rate will be further developed and then used in occupational environments. In the new sampler, airborne particles are electrically charged and then deposited on a superhydrophobic surface from which they are removed by 20 L liquid droplets. This allows concentrating the entire sample into one small droplet. A prototype sampler based on this approach was built and briefly tested in earlier research. Here it will be redesigned to achieve unprecedented sample concentration rates (to 1-2 million per minute) while allowing collection of four samples simultaneously. The new design will also improve preservation of culturability and DNA integrity of the collected microorganisms. The developed sampler will be used to assess exposures to bioaerosols in indoor office, hospital, industrial facilities, schools, agricultural environment as well as outdoors. Bioaerosol samples collected during the assessment will be analyzed by multiple methods to obtain a more comprehensive picture of bioaerosol exposures and also to evaluate compatibility of various analysis methods with the new sampling technology. Sampling in the field will range from 10 minutes to 4 hours, and performance of the new sampler will be compared to that of other samplers. Very high concentration rates will allow detecting exposures to even low microorganism concentrations and over a long time - features lacking in current bioaerosol samplers - thus improving our ability to identify the exposure risks and protect affected populations. This proposal responds to several NORA's Priority Research Agendas: National Agriculture, Forestry, and Fishing; National Services; National Transportation, Warehousing, and Utilities. Sampler's high concentration rate, plus its small footprint and low power requirements will make it easy to deploy for numerous applications not only for exposure assessment in occupational and residential environments, but also in Homeland security area. Future research will investigate application of this technology collect airborne viruses and nanoparticles.
In this project, a novel tool to estimate exposures to biological aerosols, especially to their low concentrations, will be developed. The new technology will be tested in the laboratory against other bioaerosol samplers and using different analysis techniques, and then the new sampler will be used to assess occupational exposures to bioaerosols in various environments. This novel technology will have very high concentration rate allowing to better understand workers' exposures to bioaerosols and related health effects, and to implement protective measures more effectively.
| Zhen, Huajun; Krumins, Valdis; Fennell, Donna E et al. (2018) Analysis of airborne microbial communities using 16S ribosomal RNA: Potential bias due to air sampling stress. Sci Total Environ 621:939-947 |
| Han, Taewon; Wren, Melody; DuBois, Kelsey et al. (2015) Application of ATP-based bioluminescence for bioaerosol quantification: effect of sampling method. J Aerosol Sci 90:114-123 |
| Zhen, Huajun; Krumins, Valdis; Fennell, Donna E et al. (2015) Development of a dual-internal-reference technique to improve accuracy when determining bacterial 16S rRNA:16S rRNA gene ratio with application to Escherichia coli liquid and aerosol samples. J Microbiol Methods 117:113-21 |
| Zhen, Huajun; Han, Taewon; Fennell, Donna E et al. (2013) Release of free DNA by membrane-impaired bacterial aerosols due to aerosolization and air sampling. Appl Environ Microbiol 79:7780-9 |
