CD4+ T helper cells are central regulators of adaptive immune responses. CD4+ T helper cells differentiate into several distinct subsets to provide host protection against a variety of pathogens. Each T helper cell lineage expresses characteristic transcription factors and cytokines that confer specific effector functions. The cytokine-secreting potential of effector T helper subsets requires the activation and expression of transcription factors that promote the development of each subset. Differentiation is stimulated by the cytokine microenvironment and the activation of Signal Transducer and Activator of Transcription (STAT) proteins that initiate specific genetic programs. A simple paradigm suggested the requirement for a single STAT protein activated by a cytokine that promoted the development of a specific T helper subset. However, developing Th subsets are exposed to multiple cytokines. We have recently provided evidence that the ability of Th cells to integrate signals from multiple cytokines is necessary for optimal subset development. Specifically, we demonstrated that STAT3, which clearly promotes Th17 development in isolation, promotes Th2 differentiation when it cooperates with STAT6 in programming Th2 cytokine expression by binding to many of the same loci as STAT6. In this proposal we test the hypothesis that STAT3 binds to common and unique genes among Th subsets by using chromatin immunoprecipitation and massive parallel sequencing to define the targets of STAT3 in multiple T cell subsets. These studies will define how a developing Th cell integrates multiple signals at the level of transcription factor binding and provide the basis for a new paradigm wherein a single STAT may be the key to a particular phenotype, whereas other STAT proteins cooperate to achieve optimal differentiation and ultimately immune responses.

Public Health Relevance

The development of inflammatory disease depends upon the function of subsets of T cells. This proposal examines the factors that contribute to the development of subsets that are involved in allergic and autoimmune disease. The completion of this project will result in a more detailed understanding of this process, and may identify new targets for the treatment of these diseases.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Small Research Grants (R03)
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Cellular and Molecular Immunology - B Study Section (CMIB)
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Mallia, Conrad M
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Indiana University-Purdue University at Indianapolis
Schools of Medicine
United States
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Ulrich, Benjamin J; Verdan, Felipe Fortino; McKenzie, Andrew N J et al. (2017) STAT3 Activation Impairs the Stability of Th9 Cells. J Immunol 198:2302-2309
Kaplan, Mark H (2017) The transcription factor network in Th9 cells. Semin Immunopathol 39:11-20
Olson, Matthew R; Ulrich, Benjamin J; Hummel, Sarah A et al. (2017) Paracrine IL-2 Is Required for Optimal Type 2 Effector Cytokine Production. J Immunol 198:4352-4359
Olson, Matthew R; Verdan, Felipe Fortino; Hufford, Matthew M et al. (2016) STAT3 Impairs STAT5 Activation in the Development of IL-9-Secreting T Cells. J Immunol 196:3297-304
Wu, Qiang; Gupta, Pawan Kumar; Suzuki, Hidemi et al. (2015) CD4 T Cells but Not Th17 Cells Are Required for Mouse Lung Transplant Obliterative Bronchiolitis. Am J Transplant 15:1793-1804