Excessive stimulation or suppression of physiological systems early in development leads to long-term changes in the regulatory dynamics of these systems in later life. Addiction to opiate drugs during pregnancy or exposure to stressful environments that stimulate endogenous opiate release is responsible for a wide range of changes in the brain and behavior of the offspring. The endogenous opiate system is one of many factors controlling the functional integrity of the immune system. The long-term goals of this research project are to identify the relationships between early opiate environment and later immune function using a rodent model and to determine whether perturbations of the early opiate environment induce later immune dysfunction. The experiments in this proposal are specifically designed to determine if maternal exposure to morphine influences the offsprings' cellular immune responses. This will be achieved by measuring the proliferative responses of T-cells and B- cells to mitogens, by quantitating the immunoglobulins in the offspring, by measuring the type and number of lymphocytes in the spleen, and by investigating natural killer cell activity in offspring of opiate-treated versus control animals. Other experiments will test the potential role of brain opiate receptors in immune dysfunction. Knowledge of how opiate exposure during early development influences later immune function may help design optimal strategies for treating disease in individuals born to addicted mothers.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Small Research Grants (R03)
Project #
1R03DA007365-01A1
Application #
3424225
Study Section
Sociobehavioral Subcommittee (DAAR)
Project Start
1993-07-01
Project End
1995-06-30
Budget Start
1993-07-01
Budget End
1994-06-30
Support Year
1
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Western University of Health Sciences
Department
Type
Schools of Osteopathy
DUNS #
093373694
City
Pomona
State
CA
Country
United States
Zip Code
91766
Martin, J T; Nehlsen-Cannarella, S L; Gugelchuk, G M et al. (1998) Morphine during pregnancy in the rat. Studies of cellular immunity in cross-fostered offspring. Adv Exp Med Biol 437:149-57
Martin, J T; Nehlsen-Cannarella, S L; Gugelchuk, G M et al. (1996) Prenatal morphine exposure interacts with adult stress to affect type and number of blood leucocytes. Adv Exp Med Biol 402:89-94