Reconstruction of craniofacial defects caused by fracture, trauma or degeneration is one of the major clinical challenges in the Unites States and around the world. The existing treatments have limitations and lack clinical predictability, necessitating the development of new strategies to heal, repair and regenerate the craniofacial bone tissues. The ability to regenerate new bone for craniofacial use is a significant clinical need. One approach to regenerate the tissues of interest is the seeding of autologous stem cells into a biomaterial or scaffold. These biomaterials or scaffolds support and provide cues for the cells, allowing them to grow, differentiate and secrete new extracellular matrix. It is necessary to develop new biodegradable materials, which provide an appropriate microenvironment for cell-matrix interactions that can mimic the important multi-functionality of the biological environment. This proposal focuses on development of a new approach for regenerating natural bone tissues, with emphasis on developing injectable hybrid microparticles (MPs) providing osteoconductivity, osteoinductivity and structural integrity into MPs. This will be achieved by following three specific research aims, as described below: 1. To fabricate and characterize hybrid MPs. 2. To optimize the in vitro release kinetics of growth factors. 3. To test and evaluate hybrid MPs for enhancement of osteogenesis in vitro using bone marrow stromal cells. Public Health Relevance: The relevance of this research to public health exists in the development of novel injectable hybrid microparticles that can be applied for regeneration of lost or damaged bone tissues.

National Institute of Health (NIH)
National Institute of Dental & Craniofacial Research (NIDCR)
Small Research Grants (R03)
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NIDCR Special Grants Review Committee (DSR)
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Lumelsky, Nadya L
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University of Toledo
Schools of Medicine
United States
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Mantripragada, Venkata P; Jayasuriya, Ambalangodage C (2016) Effect of dual delivery of antibiotics (vancomycin and cefazolin) and BMP-7 from chitosan microparticles on Staphylococcus epidermidis and pre-osteoblasts in vitro. Mater Sci Eng C Mater Biol Appl 67:409-417
Mantripragada, Venkata P; Jayasuriya, Ambalangodage C (2016) Bone regeneration using injectable BMP-7 loaded chitosan microparticles in rat femoral defect. Mater Sci Eng C Mater Biol Appl 63:596-608
Mantripragada, Venkata P; Jayasuriya, Ambalangodage C (2014) IGF-1 release kinetics from chitosan microparticles fabricated using environmentally benign conditions. Mater Sci Eng C Mater Biol Appl 42:506-16
Mantripragada, Venkata P; Jayasuriya, Ambalangodage C (2014) Injectable chitosan microparticles incorporating bone morphogenetic protein-7 for bone tissue regeneration. J Biomed Mater Res A 102:4276-89
Aryaei, Ashkan; Jayatissa, Ahalapitiya H; Jayasuriya, Ambalangodage C (2014) The effect of graphene substrate on osteoblast cell adhesion and proliferation. J Biomed Mater Res A 102:3282-90
Aryaei, Ashkan; Jayatissa, Ahalapitiya H; Jayasuriya, Ambalangodage C (2014) Mechanical and biological properties of chitosan/carbon nanotube nanocomposite films. J Biomed Mater Res A 102:2704-12
Jayasuriya, Ambalangodage C; Aryaei, Ashkan; Jayatissa, Ahalapitiya H (2013) ZnO nanoparticles induced effects on nanomechanical behavior and cell viability of chitosan films. Mater Sci Eng C Mater Biol Appl 33:3688-96
Mantripragada, Venkata P; Lecka-Czernik, Beata; Ebraheim, Nabil A et al. (2013) An overview of recent advances in designing orthopedic and craniofacial implants. J Biomed Mater Res A 101:3349-64
Aryaei, Ashkan; Jayasuriya, Ambalangodage C (2013) Mechanical properties of human amniotic fluid stem cells using nanoindentation. J Biomech 46:1524-30
Dreifke, Michael B; Ebraheim, Nabil A; Jayasuriya, Ambalangodage C (2013) Investigation of potential injectable polymeric biomaterials for bone regeneration. J Biomed Mater Res A 101:2436-47

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