Campylobacter rectus is a rod-shaped Gram-negative anaerobic oral bacterium that has been identified in 90% of adults with initial or established periodontitis, compared with 10% of healthy individuals and 20% of individuals with gingivitis. In addition to a clear association with periodontal disease, recent studies have highlighted the involvement of C. rectus infection in Barrett's esophoagus, oral and extraoral abcesses, inflammatory bowel disease and adverse pregnancy outcomes in humans including pre-term birth and low birth weight. Despite the frequent isolation of C. rectus from humans little is known about the molecular mechanisms of pathogenesis associated with this emerging pathogen. In order to begin an analysis of C. rectus pathogenesis, we will investigate whether invasion and invasion-related genes involved in Campylobacter jejuni pathogenesis are also responsible for C. rectus pathogenesis. In particular, we will 1) Determine if the C. rectus gene annotated as ciaB [Campylobacter invasion antigen B] is a functional homolog to C. jejuni ciaB; 2) Determine the secretion status of C. rectus ciaB and thereby establish whether a functional flagellar type II secretion system exists in C. rectus. Results gained from this small project will support future functional genomic studies of C. rectus on a larger scale to understand how C. rectus contributes to human disease.

Public Health Relevance

This research is relevant to human health because of the increasingly common association of oral Campylobacter species with periodontal disease as well as systemic diseases, including diseases of the gastrointestinal tract and adverse pregnancy outcomes. In particular we have identified a putative invasion antigen (CiaB) in Campylobacter rectus (C. rectus) and will examine its secretion and importance to pathogenesis both orally and systemically.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Small Research Grants (R03)
Project #
1R03DE023882-01A1
Application #
8968447
Study Section
NIDR Special Grants Review Committee (DSR)
Program Officer
Lunsford, Dwayne
Project Start
2015-08-01
Project End
2017-07-31
Budget Start
2015-08-01
Budget End
2016-07-31
Support Year
1
Fiscal Year
2015
Total Cost
Indirect Cost
Name
Texas A&M Agrilife Research
Department
Veterinary Sciences
Type
Schools of Veterinary Medicine
DUNS #
847205713
City
College Station
State
TX
Country
United States
Zip Code
77843