A microengineered system for the oriented capture and ex vivo culturing of an important and under-studied tissue, the intestinal crypt, will be designed and tested. The sustained culture of crypts on the array platform will be optimized by providing a lamina-propria-simulated microenvironment on the basal face of the oriented crypts, as well as by supplementing with a mixture of appropriate exogenously supplied signals. The sustained proliferation and differentiation of cells into the cell lineages found in colonic epithelium in vivo will be evaluated. The end goal will be to subject the polarized tissue to user-controlled fluidic microenvironments in a manner that recapitulates the in vivo state, and perform biological assays of living colonic crypts on the chip. The present grant will demonstrate the feasibility and potential for rationally microengineered technologies to address the specific needs of the gastrointestinal biology and disease research.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Small Research Grants (R03)
Project #
5R03EB013803-02
Application #
8290320
Study Section
Biomaterials and Biointerfaces Study Section (BMBI)
Program Officer
Hunziker, Rosemarie
Project Start
2011-07-01
Project End
2013-12-30
Budget Start
2012-07-01
Budget End
2013-12-30
Support Year
2
Fiscal Year
2012
Total Cost
$74,000
Indirect Cost
$24,000
Name
University of North Carolina Chapel Hill
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Wang, Yuli; Ahmad, Asad A; Sims, Christopher E et al. (2014) In vitro generation of colonic epithelium from primary cells guided by microstructures. Lab Chip 14:1622-31
Gracz, A D; Magness, S T (2014) Defining hierarchies of stemness in the intestine: evidence from biomarkers and regulatory pathways. Am J Physiol Gastrointest Liver Physiol 307:G260-73