Defects in sexual development in the human embryo are alarmingly common and profoundly distressing. The basis of these disorders is often a failure in the delicate network of gene regulation that controls proper development of the gonads. In mammals, the initial trigger of the cascade of gene regulation responsible for testes differentiation is the transcription factor SRY. Although a number of genes are assumed to act downstream of Sry in this cascade, no direct SRY target gene has yet been identified. This project will use a unique antibody that recognizes endogenous mouse SRY protein, recently generated in our laboratory. This antibody will be used in chromatin immunoprecipitation experiments to identify genes that are under direct control of SRY. This information will be critical in building a comprehensive picture of male sex determination and explaining the etiology of human sexual development anomalies. ? ?

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Small Research Grants (R03)
Project #
5R03HD049431-02
Application #
7067140
Study Section
Pediatrics Subcommittee (CHHD)
Program Officer
Taymans, Susan
Project Start
2005-05-19
Project End
2007-04-30
Budget Start
2006-05-01
Budget End
2007-04-30
Support Year
2
Fiscal Year
2006
Total Cost
$52,731
Indirect Cost
Name
University of Queensland
Department
Type
DUNS #
752898403
City
Brisbane
State
Country
Australia
Zip Code
4072
Wilhelm, Dagmar; Washburn, Linda L; Truong, Vy et al. (2009) Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice. Mech Dev 126:324-36
Bradford, Stephen T; Hiramatsu, Ryuji; Maddugoda, Madhavi P et al. (2009) The cerebellin 4 precursor gene is a direct target of SRY and SOX9 in mice. Biol Reprod 80:1178-88
Svingen, Terje; Wilhelm, Dagmar; Combes, Alexander N et al. (2009) Ex vivo magnetofection: a novel strategy for the study of gene function in mouse organogenesis. Dev Dyn 238:956-64
Polanco, Juan Carlos; Wilhelm, Dagmar; Mizusaki, Hirofumi et al. (2009) Functional analysis of the SRY-KRAB interaction in mouse sex determination. Biol Cell 101:55-67