Malaria is a major public health problem in China. Pyrethroid-impregnated bed nets and indoor spray with pyrethroids are the main malaria vector control tools. However, the wide use of pyrethroids has resulted in the emergence and spread of resistance in mosquito populations. Insecticide resistance has significantly hindered the efforts of malaria control in China and worldwide. This application will focus on resistance mechanisms in an important malaria vector in Southern China, Anopheles minimus C. The central hypothesis is that both knockdown resistance (kdr) and metabolic detoxification confer resistance to pyrethroids in An. minimus, and consequently, the resistance level of a mosquito to pyrethroids is contingent on mutations in kdr gene and monooxygenase activity. The kdr gene polymorphism in natural populations will be determined, and correlations between the kdr mutations, monooxygenase activity with pyrethroid resistance phenotype will be examined. This research will play an important foundation for the development of molecular- or biochemically-based resistance diagnosis tools in this important malaria vector species in China and Southeast Asia. The proposed research will contribute to building research capacity in China by training one Ph.D. student and one postdoc fellow and strengthen the molecular entomology laboratory at the Nanjing Medical University. This research will be conducted as an extension of NIH grant R01 AI050243-07.

Public Health Relevance

This application focuses on resistance mechanisms in the most important malaria vector in Southern China, Anopheles minimus C. Understanding the resistance mechanisms will play an important foundation for the development of new resistance diagnosis tools. Further, this research will contribute to building research capacity in medical entomology in China by providing research training for one Ph.D. student and one postdoc fellow, and strengthen the molecular entomology laboratory in Nanjing Medical University.

Agency
National Institute of Health (NIH)
Institute
Fogarty International Center (FIC)
Type
Small Research Grants (R03)
Project #
5R03TW008237-03
Application #
8212246
Study Section
International and Cooperative Projects - 1 Study Section (ICP1)
Program Officer
Sina, Barbara J
Project Start
2009-12-01
Project End
2013-11-30
Budget Start
2011-12-01
Budget End
2013-11-30
Support Year
3
Fiscal Year
2012
Total Cost
$58,806
Indirect Cost
$7,722
Name
University of California Irvine
Department
None
Type
Schools of Public Health
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
Chang, Xuelian; Zhong, Daibin; Lo, Eugenia et al. (2016) Landscape genetic structure and evolutionary genetics of insecticide resistance gene mutations in Anopheles sinensis. Parasit Vectors 9:228
Xu, Tielong; Zhong, Daibin; Tang, Linhua et al. (2014) Anopheles sinensis mosquito insecticide resistance: comparison of three mosquito sample collection and preparation methods and mosquito age in resistance measurements. Parasit Vectors 7:54
Qin, Qian; Li, Yiji; Zhong, Daibin et al. (2014) Insecticide resistance of Anopheles sinensis and An. vagus in Hainan Island, a malaria-endemic area of China. Parasit Vectors 7:92
Zhong, Daibin; Chang, Xuelian; Zhou, Guofa et al. (2013) Relationship between knockdown resistance, metabolic detoxification and organismal resistance to pyrethroids in Anopheles sinensis. PLoS One 8:e55475
Yu, Guo; Yan, Guiyun; Zhang, Naixin et al. (2013) The Anopheles community and the role of Anopheles minimus on malaria transmission on the China-Myanmar border. Parasit Vectors 6:264
Chen, Bin; Harbach, Ralph E; Walton, Catherine et al. (2012) Population genetics of the malaria vector Anopheles aconitus in China and Southeast Asia. Infect Genet Evol 12:1958-67
Molecular Ecology Resources Primer Development Consortium; Bonizzoni, Mariangela; Bourjea, Jérôme et al. (2011) Permanent Genetic Resources added to Molecular Ecology Resources Database 1 April 2011-31 May 2011. Mol Ecol Resour 11:935-6