This Fogarty International Research Collaboration award (FIRCA) proposal is on tsetse fly population genetics in order to support the ongoing Human African Trypanosomiasis (HAT) control activities in East Africa. The parent grant (NIAID R01Al068932, 01/01/2008 to 12/31/2012) addresses the molecular and ecological aspects of the two HAT disease belts (gambiense and rhodesiense) in Uganda with a focus on population and evolutionary genetics of tsetse flies and their parasites and endosymbionts. The co- investigator of this FIRCA, Dr. Johnson Ouma, is an experienced tsetse population geneticist who is now the head of tsetse genomic research and Deputy Director of the national Trypanosomiasis Research Center (TRC) in Kenya. Kenya is at risk of HAT outbreaks due to ongoing epidemics in neighboring Uganda and increased movement of people and cattle (known reservoirs for Trypanosoma brucei rhodesiense). Earlier tsetse control efforts in the Lake Victoria basin and in the southern Rift Valley were unsustainable and these regions rapidly became repopulated. It is unknown if the extant G. pallidipes vector populations in Lambwe originated through reinvasion from neighboring populations, or through incomplete elimination of local populations that existed below thresholds of detection. Efforts are underway once again to eliminate G. pallidipes from Lambwe valley and surrounding areas. This application has three aims to: 1) estimate rates of gene flow and degrees of genetic differentiation among G. pallidipes populations around the Lake Victoria basin and in southern Rift Valley, 2) estimate local levels of temporal genetic differentiation and dynamics of G. pallidipes populations from the Lambwe and Nguruman valleys and 3) understand the circulating trypanosome parasite diversity isolated from flies/humans and known reservoir animals in the Lambwe valley. Results will help understand the breeding pattern of G. pallidipes populations in East Africa, and thus identify populations that can serve as potential sources of immigrants into Nguruman and Lambwe. This knowledge is important to the ongoing and planned tsetse control programs and can help develop methods for inclusion or exclusion of adjacent populations to the target population during vector suppression efforts. Knowledge on parasite strains in circulation will also help better understand disease risk and epidemiology.

Public Health Relevance

Tsetse flies are vectors of African trypanosomes, agents of sleeping sickness. Kenya is under high risk for emergence of sleeping sickness. This application investigates population genetics of tsetse flies in Kenya and will benefit the ongoing vector control programs to improve their sustainability.

Agency
National Institute of Health (NIH)
Institute
Fogarty International Center (FIC)
Type
Small Research Grants (R03)
Project #
5R03TW008413-03
Application #
8284344
Study Section
International and Cooperative Projects - 1 Study Section (ICP1)
Program Officer
Sina, Barbara J
Project Start
2010-07-01
Project End
2014-06-30
Budget Start
2012-07-01
Budget End
2014-06-30
Support Year
3
Fiscal Year
2012
Total Cost
$55,955
Indirect Cost
$3,604
Name
Yale University
Department
Public Health & Prev Medicine
Type
Schools of Medicine
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Echodu, Richard; Sistrom, Mark; Bateta, Rosemary et al. (2015) Genetic diversity and population structure of Trypanosoma brucei in Uganda: implications for the epidemiology of sleeping sickness and Nagana. PLoS Negl Trop Dis 9:e0003353
International Glossina Genome Initiative (2014) Genome sequence of the tsetse fly (Glossina morsitans): vector of African trypanosomiasis. Science 344:380-6
Molecular Ecology Resources Primer Development Consortium; Aksoy, Serap; Almeida-Val, Vera Maria F et al. (2013) Permanent genetic resources added to molecular ecology resources database 1 October 2012-30 November 2012. Mol Ecol Resour 13:341-3
Wamwiri, Florence N; Alam, Uzma; Thande, Paul C et al. (2013) Wolbachia, Sodalis and trypanosome co-infections in natural populations of Glossina austeni and Glossina pallidipes. Parasit Vectors 6:232
Doudoumis, Vangelis; Alam, Uzma; Aksoy, Emre et al. (2013) Tsetse-Wolbachia symbiosis: comes of age and has great potential for pest and disease control. J Invertebr Pathol 112 Suppl:S94-103
Doudoumis, Vangelis; Tsiamis, George; Wamwiri, Florence et al. (2012) Detection and characterization of Wolbachia infections in laboratory and natural populations of different species of tsetse flies (genus Glossina). BMC Microbiol 12 Suppl 1:S3
Hyseni, Chaz; Kato, Agapitus B; Okedi, Loyce M et al. (2012) The population structure of Glossina fuscipes fuscipes in the Lake Victoria basin in Uganda: implications for vector control. Parasit Vectors 5:222
Ouma, Johnson O; Beadell, Jon S; Hyseni, Chaz et al. (2011) Genetic diversity and population structure of Glossina pallidipes in Uganda and western Kenya. Parasit Vectors 4:122
Bonomi, Angelica; Bassetti, Federico; Gabrieli, Paolo et al. (2011) Polyandry is a common event in wild populations of the Tsetse fly Glossina fuscipes fuscipes and may impact population reduction measures. PLoS Negl Trop Dis 5:e1190