This grant proposal entitled "Combination of Nanofiber Scaffolds with Gradations in Fiber Organization and Mineral Content and Adipose-derived Stem Cells for Rotator Cuff Injury Repair" addresses the challenge in engineering a tissue construct to repair rotator cuff injury. The research issues we will address in the current grant proposal are three-fold: 1) Developing novel nanofiber scaffolds that will recapitulate the native structure and composition of tendon-to-bone insertion site, 2) Regulating the behaviors of adipose- derived stem cells (ADSCs) including morphology, proliferation, migration, and differentiation using nanofiber scaffolds, and 3) Testing the efficacy of ADSCs seeded nanofiber scaffolds using a rat shoulder injury model. To address our objectives, we are taking advantage of a unique opportunity to combine expertise from two research groups at Marshall University to ensure high level of productivity during the proposed funding period. Dr. Xie's laboratory has extensive experience in fabrication of nanofiber scaffolds and examination of interaction between cells and nanofiber scaffolds including culture and differentiation of adult and embryonic stem cells. Dr. Shuler has extensive experience in the animal model and clinical treatment of rotator cuff injury. Both research groups have the necessary skills and technology required to immediately carry out the work proposed.

Public Health Relevance

Rotator cuff repair - that is, surgical attachment of the torn tendon(s) to their bony insertion(s) - to recover shoulder function is one of the most common orthopaedic surgical procedures, with tens of thousands of repairs performed each year in the United States. It is the most frequently seen shoulder condition, affecting more than 17 million individuals in the United States alone. The research program outlined here seeks to provide the foundation for the development of nanofiber-based scaffolds for promoting repair of the rotator cuff injury.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Academic Research Enhancement Awards (AREA) (R15)
Project #
1R15AR063901-01
Application #
8433133
Study Section
Musculoskeletal Tissue Engineering Study Section (MTE)
Program Officer
Wang, Fei
Project Start
2013-08-01
Project End
2016-07-31
Budget Start
2013-08-01
Budget End
2016-07-31
Support Year
1
Fiscal Year
2013
Total Cost
$293,004
Indirect Cost
$80,504
Name
Marshall University
Department
None
Type
Organized Research Units
DUNS #
036156615
City
Huntington
State
WV
Country
United States
Zip Code
25701
Jiang, J; Xie, J; Ma, B et al. (2014) Mussel-inspired protein-mediated surface functionalization of electrospun nanofibers for pH-responsive drug delivery. Acta Biomater 10:1324-32
Ma, Bing; Xie, Jingwei; Jiang, Jiang et al. (2013) Rational design of nanofiber scaffolds for orthopedic tissue repair and regeneration. Nanomedicine (Lond) 8:1459-81