Each year approximately 400,000 rotator cuff surgeries are performed in the United States. While operative techniques have improved, the re-rupture rate following rotator cuff repair remains high (30-35%). A recognized risk factor for tendon re-rupture is rotator cuff muscle degenerative atrophy. We suggest that a readily available "off the shelf" biomaterial that provides the appropriate regenerative cues by mimicking the properties of native skeletal muscle extracellular matrix could be used to regenerate damaged muscle tissue. To create a tissue mimetic material we have developed an innovative approach to capture the ECM secreted by populations of cells during growth in-vitro. This approach allows us to engineer materials that, like native tissues, are comprised entirely of cell derived constituents. The hypothesis of this research proposal is that we can use this ECM collection approach to build an engineered muscle mimetic (eMM) scaffold capable of regenerating damaged skeletal muscle. The following specific aims describe a development process in which the design of eMM scaffolds will be guided by the properties of native muscle tissue. Namely, skeletal muscle samples will be prepared and thoroughly characterized to serve as a design template (Aim 1). We will utilize our ECM collection approach to capture and organize the ECM secreted by muscle cells into an eMM scaffold with muscle mimetic composition and structure (Aim 2). We will test the regenerative performance of ECM scaffolds using a muscle defect model (Aim 3).

Public Health Relevance

A recognized risk factor for tendon re-rupture following rotator cuff surgery is shoulder muscle atrophy. The effective treatment of damaged shoulder cuff muscle could reduce the re-rupture rate, eliminate tens of thousands of repeat procedures, and greatly reduce rotator cuff related health care expenses.

Agency
National Institute of Health (NIH)
Type
Academic Research Enhancement Awards (AREA) (R15)
Project #
1R15AR064481-01A1
Application #
8626694
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Boyce, Amanda T
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Arkansas at Fayetteville
Department
Engineering (All Types)
Type
Biomed Engr/Col Engr/Engr Sta
DUNS #
City
Fayetteville
State
AR
Country
United States
Zip Code
72701