Here we will synthesize novel next generation nanoparticles of manganese or magnesium oxide or doped or composites of these with cobalt and zinc. Their characterization will be by EM and XRD and by newer infra-red and photoluminescence spectroscopy techniques we are developing. RNA molecules will be bound to the nanomaterials as studied by electron microscopy, UV/Vis, dynamic laser light scatter, and the more innovative circular dichroism (CD) spectroscopy. Ultimately we are interested in the delivery of anti-cancer RNA and thus we will test a series of novel RNA oligomer sequences designed as splice-site switching oligomers (SSO) or aptamers in comparison to siRNA or the anti-cancer poly I:C macromolecular RNA. RNA nanoconjugates will contain these RNA bound to nanomaterials alone or in combination with delivery- enhancing protamine protein or alternatively PAMAM dendrimer, and their molecular effects on splicing and the expression of B-Raf and several other important cancer targets will be determined. Finally we will investigate the effects of the RNA nanoconjugates on tumorigenicity and cancer cell signaling.

Public Health Relevance

Here we will synthesize second generation nanoparticles derived of manganese oxide (MnO) and magnesium oxide (MgO) or composites or doped versions with zinc oxide (ZnO) and cobalt. The ability of these newer nanomaterials (NMs) to bind and deliver anti-cancer RNA molecules (splice switching oligomers, aptamers, siRNA and poly inosinic:cytidilic acid) into cancer cells will be studied. The molecular effects of the novel RNA nanoconjugates (RNA NCs) created by binding RNA to these NMs will be studied for their ability to elicit anti-cancer effects at the molecular level (splicing, protein expression) and at he cellular level (cell signaling, apoptosis and tumorigenicity).

National Institute of Health (NIH)
National Cancer Institute (NCI)
Academic Research Enhancement Awards (AREA) (R15)
Project #
Application #
Study Section
Nanotechnology Study Section (NANO)
Program Officer
Fu, Yali
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Missouri State University
Other Health Professions
Other Domestic Higher Education
United States
Zip Code
Hurst, Miranda N; DeLong, Robert K (2016) Two-Dimensional Fluorescence Difference Spectroscopy to Characterize Nanoparticles and their Interactions. Sci Rep 6:33287
Dean, John M; DeLong, Robert K (2015) A high-throughput screening assay for the functional delivery of splice-switching oligonucleotides in human melanoma cells. Methods Mol Biol 1297:187-96
Bhaumik, A; Shearin, A M; Delong, R et al. (2014) Probing the Interaction at the Nano-Bio Interface Using Raman Spectroscopy: ZnO Nanoparticles and Adenosine Triphosphate Biomolecules. J Phys Chem C Nanomater Interfaces 118:18631-18639
Reyes-Reveles, Juan; Sedaghat-Herati, Reza; Gilley, David R et al. (2013) mPEG-PAMAM-G4 nucleic acid nanocomplexes: enhanced stability, RNase protection, and activity of splice switching oligomer and poly I:C RNA. Biomacromolecules 14:4108-15
Wayu, Mulugeta B; Spidle, Ryan T; Devkota, Tuphan et al. (2013) Morphology of hydrothermally synthesized ZnO nanoparticles tethered to carbon nanotubes affects electrocatalytic activity for H2O2 detection. Electrochim Acta 97:99-104
Delong, Robert K; Risor, Azure; Kanomata, Masaaki et al. (2012) Characterization of biomolecular nanoconjugates by high-throughput delivery and spectroscopic difference. Nanomedicine (Lond) 7:1851-62
Parker-Esquivel, Brooke; Flores, Kristin J; Louiselle, Daniel et al. (2012) Association of poly I:C RNA and plasmid DNA onto MnO nanorods mediated by PAMAM. Langmuir 28:3860-70
Barber, S; Abdelhakiem, M; Ghosh, K et al. (2011) Effects of nanomaterials on luciferase with significant protection and increased enzyme activity observed for zinc oxide nanomaterials. J Nanosci Nanotechnol 11:10309-19
Gann, H; Glaspell, G; Garrad, R et al. (2010) Interaction of MnO and ZnO nanomaterials with biomedically important proteins and cells. J Biomed Nanotechnol 6:37-42
Delong, Robert K; Reynolds, Christopher M; Malcolm, Yaneika et al. (2010) Functionalized gold nanoparticles for the binding, stabilization, and delivery of therapeutic DNA, RNA, and other biological macromolecules. Nanotechnol Sci Appl 3:53-63