Tumor development is a complex process significantly influenced by gene mutations and environmental factors. Chronic inflammation, as illustrated in inflammatory bowel disease (IBD), is a crucial risk factor that can predispose susceptible cells to oncogenic transformation, leading to tumor formation. Anti-inflammatory agents have been evaluated for cancer prevention. GPR4 is a pro-inflammatory pH-sensing G protein-coupled receptor (GPCR) that is activated by acidosis, a biochemical hallmark of the microenvironment of inflammatory tissues and tumors. We have recently demonstrated that activation of GPR4 by acidotic stress stimulates the expression of inflammatory genes in vascular endothelial cells (ECs) and also augments leukocyte-EC adhesion. Our preliminary results further demonstrate that GPR4 increases inflammatory responses in a mouse model of inflammatory bowel disease. In this project, we will investigate the role of GPR4 in intestinal inflammation and cancer using GPR4 knockout mice and pharmacological GPR4 inhibitors. We will employ widely accepted protocols using dextran sulfate sodium (DSS) to induce inflammatory bowel disease and using DSS in combination with azoxymethane (AOM) to induce colitis-associated colorectal cancer in wild-type and GPR4 knockout mice and determine how GPR4 deficiency modulates intestinal inflammation and neoplasia. We will also examine the therapeutic effects of the GPR4 inhibitor on regulating intestinal inflammation and carcinogenesis in the IBD mouse model.

Public Health Relevance

Acidosis is an important stress factor involved in a variety of human diseases such as inflammation, cancer, diabetes, ischemia, lung diseases and kidney diseases. We have recently shown that the GPR4 receptor is a functional acid sensor in vascular endothelial cells, and activation of GPR4 by acidosis induces a broad inflammatory response in endothelial cells and enhances endothelial cell-leukocyte adhesion. Here we propose to investigate the role for GPR4 in intestinal inflammation and colitis-associated colorectal cancer. We will also assess the therapeutic value of the small molecule modulators of GPR4 for the treatment and prevention of inflammatory bowel disease and colorectal cancer.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Academic Research Enhancement Awards (AREA) (R15)
Project #
1R15DK109484-01
Application #
9099155
Study Section
Special Emphasis Panel (ZRG1-OBT-H (81)A)
Program Officer
Perrin, Peter J
Project Start
2016-09-16
Project End
2019-08-31
Budget Start
2016-09-16
Budget End
2019-08-31
Support Year
1
Fiscal Year
2016
Total Cost
$436,300
Indirect Cost
$136,300
Name
East Carolina University
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
607579018
City
Greenville
State
NC
Country
United States
Zip Code
27858
Yang, Li V (2017) Tumor Microenvironment and Metabolism. Int J Mol Sci 18:
Sanderlin, Edward J; Leffler, Nancy R; Lertpiriyapong, Kvin et al. (2017) GPR4 deficiency alleviates intestinal inflammation in a mouse model of acute experimental colitis. Biochim Biophys Acta 1863:569-584
Justus, Calvin R; Sanderlin, Edward J; Dong, Lixue et al. (2017) Contextual tumor suppressor function of T cell death-associated gene 8 (TDAG8) in hematological malignancies. J Transl Med 15:204
Dong, Lixue; Krewson, Elizabeth A; Yang, Li V (2017) Acidosis Activates Endoplasmic Reticulum Stress Pathways through GPR4 in Human Vascular Endothelial Cells. Int J Mol Sci 18: