Maternal effects comprise the influences on offspring phenotype resulting from the environmental experience of the mother. An important source of maternal effects is prenatal stress, which can have profound effects on the resulting phenotype of the offspring, and in humans, can lead to impaired growth and serious mental health and physical disorders. Stress results in the secretion of stress hormones that, while beneficial in th short-term, can lead to deterioration in the health of an individual if they remain elevated over a extended period. The particular focus in our proposed studies will be the prenatal maternal stress that ensues when immune responses of females are experimentally induced during reproduction. Our proposed research employs a wild bird model that provides an excellent alternative model to evaluate maternal effects of prenatal stress because: (i) the endocrine systems of birds are similar to those of mammals, (ii) bird embryos are readily amenable to experimental manipulation within eggs, and (iii) wild birds are easily captured and manipulated in their natural environment, allowing inferences about the role of maternal stress to be made in a realistic evolutionary context. Our basic approach for imposing prenatal stress will be to inject females with lipopolysaccharides, a nonliving, nonpathogenic, molecular component of the outer membrane of the cell wall of gram-negative bacteria.
Our first aim i s to determine whether experimental stimulation of the maternal immune system leads to increased production of the avian stress hormone, corticosterone, that is transferred to the developing embryo in the egg.
Aim 2 lies at the heart of the proposed research, with its focus on determining the effects on offspring phenotype of the experimental induction of a stressful prenatal female immune response. Demonstration of such maternal effects as they shape offspring phenotype will provide an unparalleled understanding of how maternal stress is translated into differences in offspring size, health state, and immune function. Finally, our third aim is to determine whether maternal effects arising from prenatal stress can be adaptive, as indicated by beneficial sex-specific effects on offspring fitness. The proposed research will meet a primary goal of the AREA program in that it will expose undergraduate students to research that combines both field and laboratory work in a hypothetico-deductive framework to address important biomedical questions in a natural setting.

Public Health Relevance

This application seeks to determine the short- and long-term effects on offspring of the stress that occurs when a mother's immune system is activated prenatally. Prenatal maternal stress can have profound effects on the size, health, and immunity of offspring, and in humans can lead to impaired growth and serious mental health and physical disorders. We will conduct studies on the hormonal basis of stress-mediated maternal effects and evaluate the costs and benefits of these effects on offspring in an adaptive framework.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Academic Research Enhancement Awards (AREA) (R15)
Project #
1R15HD076308-01
Application #
8496930
Study Section
Special Emphasis Panel (ZRG1-BBBP-R (91))
Program Officer
Raju, Tonse N
Project Start
2013-06-01
Project End
2016-05-31
Budget Start
2013-06-01
Budget End
2016-05-31
Support Year
1
Fiscal Year
2013
Total Cost
$411,576
Indirect Cost
$116,593
Name
Illinois State University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
001898142
City
Normal
State
IL
Country
United States
Zip Code
61790
Strange, Meghan S; Bowden, Rachel M; Thompson, Charles F et al. (2016) Pre- and Postnatal Effects of Corticosterone on Fitness-Related Traits and the Timing of Endogenous Corticosterone Production in a Songbird. J Exp Zool A Ecol Genet Physiol 325:347-59
Bowers, E K; Thompson, C F; Sakaluk, S K (2016) Within-female plasticity in sex allocation is associated with a behavioural polyphenism in house wrens. J Evol Biol 29:602-16
Bowers, E Keith; Grindstaff, Jennifer L; Soukup, Sheryl Swartz et al. (2016) Spring temperatures influence selection on breeding date and the potential for phenological mismatch in a migratory bird. Ecology 97:2880-2891
Bowers, Emerson Keith; Sakaluk, Scott K; Thompson, Charles F (2016) No effect of blood sampling or phytohaemagglutinin injection on postfledging survival in a wild songbird. Ecol Evol 6:3107-14
Bowers, E Keith; Bowden, Rachel M; Thompson, Charles F et al. (2016) Elevated corticosterone during egg production elicits increased maternal investment and promotes nestling growth in a wild songbird. Horm Behav 83:6-13
Barnett, Craig A; Suzuki, Toshitaka N; Sakaluk, Scott K et al. (2015) Mass-based condition measures and their relationship with fitness: in what condition is condition? J Zool (1987) 296:1-5
Bowers, E Keith; Forsman, Anna M; Masters, Brian S et al. (2015) Increased extra-pair paternity in broods of aging males and enhanced recruitment of extra-pair young in a migratory bird. Evolution 69:2533-41
Bowers, E K; White, A; Lang, A et al. (2015) Eggshell porosity covaries with egg size among female House Wrens (Troglodytes aedon) but is unrelated to incubation onset and egg-laying order within clutches. Can J Zool 93:421-425
Bowers, E Keith; Bowden, Rachel M; Sakaluk, Scott K et al. (2015) Immune activation generates corticosterone-mediated terminal reproductive investment in a wild bird. Am Nat 185:769-83
Bowers, E Keith; Thompson, Charles F; Sakaluk, Scott K (2015) Persistent sex-by-environment effects on offspring fitness and sex-ratio adjustment in a wild bird population. J Anim Ecol 84:473-86

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