The long term outcome of this project will be a highly sensitive, rapid, robust analytical technique that can be broadly employed by neuroscientists and neurologists to monitor real-time changes in adenosine and related molecules in the brain. This research will allow further study of the involvement of these compounds in the neuronal injury induced by stroke or epilepsy, in neurodegenerative disorders such as Parkinson's disease, Huntington's disease or Alzheimer's disease and in psychiatric disorders such as schizophrenia and addictive behaviour. The basis of this analytical technique is the dramatic fluorescence enhancement of an adenosine derivative by a buffer containing -cyclodextrin. The fluorescence enhancement of the derivative will allow analysis of biological samples by capillary electrophoresis with laser-induced fluorescence detection. Reaction kinetics and fluorescence will be optimized, followed by analytical and pharmacological validation of the technique.

Public Health Relevance

The long term outcome of this project will be a highly sensitive, rapid, robust analytical technique that can be broadly employed by neuroscientists and neurologists to monitor real-time changes in Adenosine and related molecules in the brain. This will allow to further study the involvement of these compounds in the neuronal injury induced by stroke or epilepsy, in neurodegenerative disorders such as Parkinson's disease, Huntington's disease or Alzheimer's disease and in psychiatric disorders such as schizophrenia and addictive behavior.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Academic Research Enhancement Awards (AREA) (R15)
Project #
1R15NS074422-01A1
Application #
8291167
Study Section
Molecular Neurogenetics Study Section (MNG)
Program Officer
Stewart, Randall R
Project Start
2012-05-01
Project End
2014-04-30
Budget Start
2012-05-01
Budget End
2014-04-30
Support Year
1
Fiscal Year
2012
Total Cost
$407,599
Indirect Cost
$110,402
Name
University of Alaska Fairbanks
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
615245164
City
Fairbanks
State
AK
Country
United States
Zip Code
99775