There is considerable interest in understanding the mechanisms and function of mosquito innate immunity, with recent research in this area identifying important immune genes and metabolic pathways that appear to influence the ability of mosquitoes to transmit malaria. However nearly all such studies have been conducted under highly controlled lab environments, which is not what mosquitoes and Plasmodium parasites experience in the field. Natural malaria transmission occurs across a temperature range of 15-35 0 C, with the potential for considerable variation in conditions across temporal and spatial scales. We wish to test the hypothesis that temperature has significant impacts on mosquito immune function and vector competence, and to explore whether the insights gained under one set of controlled conditions are sufficient to understand variation in mosquito resistance/refractoriness in nature. We argue that mosquito immune function should be affected by environmental temperature because mosquitoes are small-bodied and cold-blooded. In other insect-parasite systems temperature has profound effects on parasite development and overall host resistance. Whether such effects occur with mosquitoes and malaria and if so, whether these effects are due to impacts of temperature on the parasite, on host immunity, or some combination of both, remains unclear. If temperature does affect mosquito immune function, then temperature may be a potent environmental parameter describing variation in mosquito resistance / refractoriness in natural vector populations and might also explain the disparities in immune responses to different Plasmodium parasites (in particular between some rodent malaria parasites and P. falciparum). More fundamentally, if mosquito immune response exhibits thermal sensitivity, the current approach of outlining immunological mechanism under standard lab conditions is insufficient for understanding natural or transgenic vector competence.
Current understanding of mosquito innate immunity derives almost exclusively from studies conducted under standard laboratory conditions. Yet in nature, mosquitoes and parasites interact across a range of environments. We wish to test the hypothesis that environmental temperature significantly shapes mosquito immune function and determine whether notional resistance mechanisms revealed in the lab can explain variation in mosquito resistance to malaria in the field.
|Cator, Lauren J; Pietri, Jose E; Murdock, Courtney C et al. (2015) Immune response and insulin signalling alter mosquito feeding behaviour to enhance malaria transmission potential. Sci Rep 5:11947|
|Moller-Jacobs, Lillian L; Murdock, Courtney C; Thomas, Matthew B (2014) Capacity of mosquitoes to transmit malaria depends on larval environment. Parasit Vectors 7:593|
|Murdock, Courtney C; Blanford, Simon; Hughes, Grant L et al. (2014) Temperature alters Plasmodium blocking by Wolbachia. Sci Rep 4:3932|
|Murdock, Courtney C; Blanford, Simon; Luckhart, Shirley et al. (2014) Ambient temperature and dietary supplementation interact to shape mosquito vector competence for malaria. J Insect Physiol 67:37-44|
|Brock, Patrick M; Murdock, Courtney C; Martin, Lynn B (2014) The history of ecoimmunology and its integration with disease ecology. Integr Comp Biol 54:353-62|
|Murdock, Courtney C; Moller-Jacobs, Lillian L; Thomas, Matthew B (2013) Complex environmental drivers of immunity and resistance in malaria mosquitoes. Proc Biol Sci 280:20132030|
|Cator, Lauren J; George, Justin; Blanford, Simon et al. (2013) 'Manipulation' without the parasite: altered feeding behaviour of mosquitoes is not dependent on infection with malaria parasites. Proc Biol Sci 280:20130711|
|Murdock, Courtney C; Paaijmans, Krijn P; Cox-Foster, Diana et al. (2012) Rethinking vector immunology: the role of environmental temperature in shaping resistance. Nat Rev Microbiol 10:869-76|
|Murdock, C C; Paaijmans, Krijn P; Bell, Andrew S et al. (2012) Complex effects of temperature on mosquito immune function. Proc Biol Sci 279:3357-66|