The connection subdomain and RNase H domain of HIV-1 RT have recently been implicated in the emergence of HIV-1 strains that are resistant to NRTI and NNRTI drugs. Site-specific mutations in the connection subdomain and RNase H domain reportedly affect substrate or NRTI binding affinity and other polymerase-related properties such as processivity, suggesting that definitive long-distance cross-talk occurs between the polymerase domain and these two distantly located structural units of HIV-1 RT. Numerous mechanisms, including a reduction in RNase H cleavage activity, which decreased template switching, and improper positioning of template-primer substrate have been proposed to explain the drug-resistant mutations. Based on the three-dimensional (3D) structure of HIV-1 RT, we propose that hydrophobic residues at the C-terminal region of the RNase H domain interacting with the C-terminal connection subdomain may be structurally important in subunit dimerization;any mutational changes in these regions may affect dimer stability and the substrate binding affinity of HIV-1 RT. We propose to investigate the implications of mutations of hydrophobic residues at the interface of the connection subdomain and RNase H domain of HIV-1 RT with respect to dimer stability and drug sensitivity of the enzyme. We will also include clinically relevant drug-resistant mutations that emerge in connection and RNase H under drug pressure.

Public Health Relevance

HIV-1 reverse transcriptase remains a primary therapeutic target for anti-HIV drug development. However, the emergence of drug-resistant HIV-1 strains limits the effectiveness of drugs and presents new challenges in developing effective drugs. Lack of clear understanding of underlying mechanisms involved in the appearance of drug-resistant strains has further complicated the development of anti-HIV drugs. Recent studies have shown that not only the polymerase domain, but the entire enzyme molecule, including the RNase H domain, contribute to emergence of drug-resistant strains. This study will analyze naturally occurring mutational changes in both the connection subdomain and RNase H domain of HIV-1 RT, as well as hydrophobic residues at the interface of connection and RNase H with regard to their implications for drug resistance and dimerization of the enzyme.

National Institute of Health (NIH)
Exploratory/Developmental Grants (R21)
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AIDS Discovery and Development of Therapeutics Study Section (ADDT)
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Fitzgibbon, Joseph E
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Rutgers University
Schools of Medicine
United States
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