The primary goal of this proposal is to understand how allosteric conformational properties of the most common, type 1 fimbrial adhesin of E. coli defines its role in the pathogenesis of urinary tract infections. FimH is an adhesive subunit of th type 1 fimbriae and is one of the major factors in the ability of E. coli to cause urinary tract infection. We determined that the mannose-binding lectin domain of FimH can assume two conformational states - with a high- and low-affinity towards terminally-exposed mannosyl residues. The conformational shift in FimH is highly dynamic in nature and is the basis of the ability of FimH to mediate shear-enhanced bacterial adhesion. We intend to analyze the conformational switch in FimH in the context of the immune response against the adhesive protein.

Public Health Relevance

The primary goal of this proposal is to understand how the ability of most common Escherichia coli adhesin, FimH, to dynamically change its structure is affecting the type of antibodies that are produced against it. We intend to perform a comprehensive analysis of the molecular and protective properties of the antibodies in context of the their specificity and inhibition of the bacterial cell adhesion and pathogenicity.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21AI103846-01A1
Application #
8588239
Study Section
Special Emphasis Panel (ZRG1-IDM-A (80))
Program Officer
Korpela, Jukka K
Project Start
2013-07-05
Project End
2015-06-30
Budget Start
2013-07-05
Budget End
2014-06-30
Support Year
1
Fiscal Year
2013
Total Cost
$217,845
Indirect Cost
$76,845
Name
University of Washington
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195