We propose to use a combination of two powerful techniques: aptamer selection (systematic evolution of ligands by exponential enrichment;SELEX) and in vitro compartmentalization (IVC), to develop a new method of generating libraries of functionalized nanoparticles and that can be screened directly for function. While the goal of this application is to develop a novel system for the direct identification of targeted nanoparticles which localize to human tumors, we will develop a platform technology which extends beyond the detection and treatment of cancer and other diseases and will result in the generation of a novel class of capture agents that will find use in a variety of applications and fields of research.

Public Health Relevance

We are engineering a new platform technology that will be of great utility in a variety of different fields for diagnostic, therapeutic and research applications. We will use this technology to develop a new class of targeting agents that are specific for cancer cells.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21CA157366-02
Application #
8319439
Study Section
Special Emphasis Panel (ZCA1-SRLB-Q (J1))
Program Officer
Ossandon, Miguel
Project Start
2011-08-12
Project End
2014-07-31
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
2
Fiscal Year
2012
Total Cost
$176,055
Indirect Cost
$70,633
Name
Albert Einstein College of Medicine
Department
Biochemistry
Type
Schools of Medicine
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Padlan, Camille S; Malashkevich, Vladimir N; Almo, Steve C et al. (2014) An RNA aptamer possessing a novel monovalent cation-mediated fold inhibits lysozyme catalysis by inhibiting the binding of long natural substrates. RNA 20:447-61
Trevino, Simon G; Levy, Matthew (2014) High-throughput bead-based identification of structure-switching aptamer beacons. Chembiochem 15:1877-81
Wengerter, Brian C; Katakowski, Joseph A; Rosenberg, Jacob M et al. (2014) Aptamer-targeted antigen delivery. Mol Ther 22:1375-87