The practical goals of this study will be the development and optimization of a serum free cryoprotection medium with minimum levels of cryoprotectants. We intend to pursue these studies using a hypothesis-driven, mechanism-based experimental approach, i.e., the viability of the cryopreserved adult stem cells (ASCs), in the undifferentiated, adipocyte- or osteoblast- differentiated states, can be improved by increasing the pre-freeze expression of heat shock proteins (HSPs).
This proposal will determine the link between the expression of heat shock proteins and pre-freeze (control) and post-freeze (cryopreservation) potential (osteogenic and adipogenic) of human adipose tissue derived adult stem cells (ASCs).
|Mehta, Nishir; Shaik, Shahensha; Devireddy, Ram et al. (2018) Single-Cell Analysis Using Hyperspectral Imaging Modalities. J Biomech Eng 140:|
|Shaik, Shahensha; Wu, Xiying; Gimble, Jeffrey et al. (2018) Effects of Decade Long Freezing Storage on Adipose Derived Stem Cells Functionality. Sci Rep 8:8162|
|Devireddy, Ram V (2013) BIOPRESERVATION: HEAT/MASS TRANSFER CHALLENGES AND BIOCHEMICAL/GENETIC ADAPTATIONS IN BIOLOGICAL SYSTEMS. Heat Transf Res 44:245-272|