The mammalian genomes express a large number of long noncoding RNAs (lncRNAs). Only a handful of them, however, have been studied. The known lncRNAs have diverse roles in the cell, mostly in the nucleus, such as chromatin remodeling, transcription, and RNA processing. Adipogenesis is the process that generates adipocytes from precursor cells, which have many implications for human diseases, such as diabetes and cardiovascular diseases. While regulation of protein-coding genes in adipogenesis has been well studied, the role of lncRNAs is completely unclear. We have sequenced the transcriptomes of human and mouse cells undergoing adipogenesis to an unprecedented depth. Our preliminary analysis indicates that a set of lncRNAs are highly regulated during adipogenesis, suggesting their functional roles in the process. In this grant, we plan to examine known and novel lncRNAs that are regulated in adipogenesis for their roles in differentiation of precursor cells into mature adipocytes.

Public Health Relevance

Adipocytes have many implications for human diseases. Aberration in fat cell number leads to obesity, which is rapidly becoming a serious public health problem around the world, and is related to other common diseases, such as type II diabetes and cardiovascular diseases. Adipogenesis is the process that generates adipocytes from precursor cells, which involves a cascade of signaling events leading to regulation of genes that play roles in cell proliferation and differentiation. In this grant, we will identify and examine long noncoding RNAs (lncRNAs), which are increasingly found to be associated with various key functions in the cell. However, their roles in adipogenesis are completely unclear.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Exploratory/Developmental Grants (R21)
Project #
6R21DK094207-03
Application #
8716868
Study Section
Cellular Aspects of Diabetes and Obesity Study Section (CADO)
Program Officer
Haft, Carol R
Project Start
2011-12-05
Project End
2013-11-30
Budget Start
2013-07-01
Budget End
2013-11-30
Support Year
3
Fiscal Year
2013
Total Cost
$97,674
Indirect Cost
$36,244
Name
Rutgers University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
078795851
City
Newark
State
NJ
Country
United States
Zip Code
07103
Hoque, Mainul; Ji, Zhe; Zheng, Dinghai et al. (2013) Analysis of alternative cleavage and polyadenylation by 3' region extraction and deep sequencing. Nat Methods 10:133-9