High-throughput analysis of cell response to chemical libraries. The study of cell behavior in response to various natural or synthetic stimuli has applications in a wide variety of fields ranging from basic biological studies to drug discovery. It is well known that the cellular microenvironment is critical in determining a cell's subsequent behavior. Therefore, it is critical to develop approaches in which the cellular microenvironment can be modulated in a rapid manner. Many high-throughput approaches currently in practice, however, require expensive equipment, large sample volumes, long incubation times and/or extensive expertise. Microscale technologies provide powerful tools to fabricate devices for high-throughput testing of the cellular response to various chemicals. The development of simple systems that can be used to perform high throughput screening is of great benefit. The overall goal of the proposed research is to develop a microarray for the high-throughput analysis of cell behavior in response to chemicals in their microenvironment. This proposal aims to make a significant impact on the ability to screen and understand cellular behavior and to result in a significant scientific impact.

Public Health Relevance

We aim to develop microarray systems incorporated with microwells for high-throughput analysis of cell response to chemical libraries.

National Institute of Health (NIH)
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Exploratory/Developmental Grants (R21)
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Instrumentation and Systems Development Study Section (ISD)
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Korte, Brenda
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Brigham and Women's Hospital
United States
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Wang, Zongjie; Calpe, Blaise; Zerdani, Jalil et al. (2016) High-throughput investigation of endothelial-to-mesenchymal transformation (EndMT) with combinatorial cellular microarrays. Biotechnol Bioeng 113:1403-12
Gaharwar, Akhilesh K; Patel, Alpesh; Dolatshahi-Pirouz, Alireza et al. (2015) Elastomeric nanocomposite scaffolds made from poly(glycerol sebacate) chemically crosslinked with carbon nanotubes. Biomater Sci 3:46-58
Gaharwar, Akhilesh K; Patel, Alpesh; Dolatshahi-Pirouz, Alireza et al. (2015) Elastomeric nanocomposite scaffolds made from poly (glycerol sebacate) chemically crosslinked with carbon nanotubes. Biomater Sci 3:45-68
Gaharwar, Akhilesh K; Mihaila, Silvia M; Kulkarni, Ashish A et al. (2014) Amphiphilic beads as depots for sustained drug release integrated into fibrillar scaffolds. J Control Release 187:66-73
Gaharwar, Akhilesh K; Peppas, Nicholas A; Khademhosseini, Ali (2014) Nanocomposite hydrogels for biomedical applications. Biotechnol Bioeng 111:441-53
Bae, Hojae; Chu, Hunghao; Edalat, Faramarz et al. (2014) Development of functional biomaterials with micro- and nanoscale technologies for tissue engineering and drug delivery applications. J Tissue Eng Regen Med 8:1-14
Gaharwar, Akhilesh K; Nikkhah, Mehdi; Sant, Shilpa et al. (2014) Anisotropic poly (glycerol sebacate)-poly (?-caprolactone) electrospun fibers promote endothelial cell guidance. Biofabrication 7:015001
Tamayol, Ali; Akbari, Mohsen; Annabi, Nasim et al. (2013) Fiber-based tissue engineering: Progress, challenges, and opportunities. Biotechnol Adv 31:669-87
Patel, Alpesh; Gaharwar, Akhilesh K; Iviglia, Giorgio et al. (2013) Highly elastomeric poly(glycerol sebacate)-co-poly(ethylene glycol) amphiphilic block copolymers. Biomaterials 34:3970-3983
Sant, Shilpa; Iyer, Dharini; Gaharwar, Akhilesh K et al. (2013) Effect of biodegradation and de novo matrix synthesis on the mechanical properties of valvular interstitial cell-seeded polyglycerol sebacate-polycaprolactone scaffolds. Acta Biomater 9:5963-73

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