The goal of this proposal is to develop a tissue hydration imaging system based on THz and NIR illumination to spatially and temporally map the hydration state of burn wounds and correlate these measurements with burn severity. Burn severity is typically assessed with visual and tactile feedback from the surgeon and diagnostic accuracy varies widely based on the clinical presentation and the surgeon's experience. In a large number of burn centers complementary assessments are made by burn wound blood perfusion measured with Laser Doppler Imaging (LDI) and vital dye based systems. These techniques are based on blood perfusion and do not provide immediate assessment of burn wound severity due to the evolving response to the burn injury. This limitation prevents the use of these techniques during the first 48 hours following injury leading to a delay in optimal management. Preliminary in vivo results obtained by our group in rats suggest that hydration imaging acquired with THz and NIR illumination may provide information to non-invasively assess burn severity within the first few hours of injury. This information may allow the burn surgeon to rapidly differentiate between partial and full thickness injury. THz and NIR illumination are eye safe, have very low photon energy, and are extremely sensitive to hydration changes secondary to edema which is part of the tissue response to thermal injury. This study will begin with the construction of a scanned beam, THz imaging system capable of non-contact, imaging of a 10 cm x 10 cm square area in less than 30 seconds. A companion NIR imaging system tuned to the peak absorption wavelength of water will be developed and used for comparison. We will also provide additional information regarding wound hydration. Year 2 will encompass extensive animal work in rats (N = 20) and the capability of these imaging systems for burn severity diagnosis will be evaluated against clinical and histopathological diagnosis.

Public Health Relevance

This project will develop a new imaging tool for non-invasive hydration measurements of burn wounds that can be used for burn severity assessment. Current burn imaging systems image blood only and cannot be used effectively within the first 48 hours after injury. The proposed work will develop imaging systems to accurately image and assess burn wound severity and will be evaluated on in vivo rat models

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21EB015084-02
Application #
8646918
Study Section
Biomedical Imaging Technology Study Section (BMIT)
Program Officer
Conroy, Richard
Project Start
2013-04-10
Project End
2015-03-31
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
2
Fiscal Year
2014
Total Cost
$179,262
Indirect Cost
$58,012
Name
University of California Los Angeles
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095