? ? The goal of this research is to develop high-frequency three-dimensional (3D) ultrasound elasticity imaging which can detect the onset of keratoconus, monitor progression of this disease, and for late stage treatment aid corneal transplant surgery. High-frequency ultrasound elasticity imaging has mainly been used with two- dimensional (2D) motion tracking of tissue deformation. Unfortunately 2D elasticity imaging is severely restricted to well controlled tissue deformation in order to prevent artifacts caused by out-of-plane motion (motion perpendicular to the imaging plane). These restrictions prevent imaging of the full cornea and so limit the usefulness of 2D elasticity imaging for examining keratoconus. We can mitigate out-of-plane motion artifacts by developing high-frequency 3D motion tracking. To do this we will modify high-frequency ultrasound confocal imaging to be used for 3D imaging. We will adapt algorithms used for low-frequency 3D speckle tracking in breast tissue to high-frequency imaging of corneal tissue. Finally we will test these methods on cadaver pig eye globes. For some of these, stiffness regions in the cornea will be modified with chemicals like formalin and collagenase to simulate abnormal tissue. These techniques, developed for examining keratoconus, can be used to applications outside of ophthalmology such as detection and characterization of skin cancer for example. Keratoconus is a disease that weakens the cornea resulting in loss of corneal shape which in turn distorts the eye's ability to focus. We are developing high resolution three-dimensional ultrasound elasticity imaging which can detect the onset of keratoconus, monitor progression of this disease, and in its late stage aid corneal transplant surgery. ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21EY018727-01
Application #
7356911
Study Section
Special Emphasis Panel (ZRG1-SBIB-U (91))
Program Officer
Shen, Grace L
Project Start
2008-01-01
Project End
2009-11-30
Budget Start
2008-01-01
Budget End
2008-11-30
Support Year
1
Fiscal Year
2008
Total Cost
$208,492
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Winterroth, Frank; Kato, Hiroko; Kuo, Shiuhyang et al. (2014) High-frequency ultrasonic imaging of growth and development in manufactured engineered oral mucosal tissue surfaces. Ultrasound Med Biol 40:2244-51
Hollman, Kyle W; Shtein, Roni M; Tripathy, Sakya et al. (2013) Using an ultrasound elasticity microscope to map three-dimensional strain in a porcine cornea. Ultrasound Med Biol 39:1451-9
Winterroth, Frank; Hollman, Kyle W; Kuo, Shiuhyang et al. (2013) Characterizing morphology and nonlinear elastic properties of normal and thermally stressed engineered oral mucosal tissues using scanning acoustic microscopy. Tissue Eng Part C Methods 19:345-51
Hollman, Kyle W; Tripathy, Sakya; Kim, Kang (2011) Three-dimensional mapping of strain in ex vivo porcine cornea with an ultrasound elasticity microscope. Conf Proc IEEE Eng Med Biol Soc 2011:8503-6
Winterroth, Frank; Hollman, Kyle W; Kuo, Shiuhyang et al. (2011) Comparison of scanning acoustic microscopy and histology images in characterizing surface irregularities among engineered human oral mucosal tissues. Ultrasound Med Biol 37:1734-42
Winterroth, Frank; Lee, Junho; Kuo, Shiuhyang et al. (2011) Acoustic microscopy analyses to determine good vs. failed tissue engineered oral mucosa under normal or thermally stressed culture conditions. Ann Biomed Eng 39:44-52
Winterroth, Frank; Hollister, Scott J; Feinberg, Stephen E et al. (2011) Non-linear stress-strain measurements of ex vivo produced oral mucosal equivalent (EVPOME) compared to normal oral mucosal and skin tissue. Conf Proc IEEE Eng Med Biol Soc 2011:286-9