Antiviral therapy is effective in preventing AIDS in HIV-infected people. However, an increasing number of HIV infected individuals suffer or even die of diseases other than AIDS including cardiovascular diseases. The biology behind these clinical observations is not well understood, although both anti-retroviral therapy (ART) dependent and independent mechanisms appear to be involved. Here we plan to study a key mechanism by which HIV can cause endothelial activation and dysfunction, which are believed to precede atherosclerotic processes and also lymphatic vessel biology. Nef is known to play a pivotal role in HIV pathogenesis and to mediate its own transfer from T cells to bystander cells. In this proposal, we postulate that HIV-Nef can efficiently be transferred from T cells to other blood cells including vascular endothelial cells, which are in steady contact with flowing blood and lymph fluid. Indeed, we can show that transfer of Nef leads to endothelial cell activation and cell death which can be effectively inhibited by antioxidants, suggesting that antioxidants could be added to the treatment regimen of HIV-infected people on ART. Based on our preliminary data that HIV dependent endothelial activation is explained by transfer of Nef from blood cells to coronary arterial endothelial cells, we further hypothesize that transfer of Nef to vascular cells may lead o cardiovascular dysfunction and pathology of the cardiovascular system. Understanding the cellular trafficking and the signal transduction of Nef in the vascular wall is important because these processes involve all vascular cells, with the endothelium playing an important regulatory role. Thus, determining the cellular mechanism of Nef-induced vascular pathology in vascular bystander cells can help to identify HIV-Nef as a novel target for preventing and treating HIV-associated diseases.

Public Health Relevance

HIV patients on anti-retroviral therapy (ART) bear an increased risk of diseases linked to the vascular endothelium, the inner lining of our blood vessels. Here we address the novel hypothesis that Nef, an early HIV protein, which is produced in reservoir cells even when the virus is treated with ART, is transferred to the endothelium to induce cell death and vascular dysfunction. If this study is successful it may generate insight for improving treatment of HIV in people.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21HL120390-01A1
Application #
8717305
Study Section
Special Emphasis Panel (ZRG1-AARR-K (58))
Program Officer
Mcdonald, Cheryl
Project Start
2014-04-01
Project End
2016-03-31
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
1
Fiscal Year
2014
Total Cost
$234,000
Indirect Cost
$84,000
Name
Indiana University-Purdue University at Indianapolis
Department
Physiology
Type
Schools of Medicine
DUNS #
603007902
City
Indianapolis
State
IN
Country
United States
Zip Code
46202