Human pluripotent stem cells are promising and potentially unlimited cell source for regenerative medicine;however, tumor formation is of major concern for cell therapy with pluripotent stem cells. Residual undifferentiated stem cells in preparations of stem cell derivatives, including cardiomyocyte differentiation cultures, have potential to proliferate and form teratoma upon transplantation, posing a major hurdle for safe cell therapy. Careful assessment of differentiation cultures and development of strategies to remove undifferentiated stem cells will facilitate qualification of final stem cell products for therapeutic use. In this study, we propose to develop sensitive, nanotechnology-based assays to detect residual undifferentiated cells, characterize cardiomyocyte differentiation cultures at various stages for their propensities to form teratomas, and evaluate strategies to selectively remove residual undifferentiated cells. Technology established in this study will be valuable for the development of novel cell therapy using human pluripotent stem cells.

Public Health Relevance

Human pluripotent stem cells have extensive proliferative capacity and can differentiate into many cell types including functional cardiomyocytes, representing promising cell source for regenerative medicine;however, teratoma formation is one of major safety concerns for clinical application. This study aims to develop sensitive nanotechnology-based assays to detect residual undifferentiated cells in differentiation cultures, characterize cardiomyocyte differentiation cells and evaluate strategies to prevent teratoma formation. Findings from this study will facilitate clinical translation of human pluripotent stem cells.

Agency
National Institute of Health (NIH)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21HL123928-01
Application #
8752641
Study Section
Bioengineering, Technology and Surgical Sciences Study Section (BTSS)
Program Officer
Danthi, Narasimhan
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Emory University
Department
Pediatrics
Type
Schools of Medicine
DUNS #
City
Atlanta
State
GA
Country
United States
Zip Code
30322