This proposal aims to develop a nanoscale electrochemical sensor that will be used to determine the role gliotransmitters play as intercellular signaling molecules in the central nervous system. The sensing platform will combine the chemical specificity afforded by electrochemical, aptamer-based sensors with the exquisite spatiotemporal resolution afforded by nanoscale electrodes and will be amenable to use with patch clamp instrumentation.
The aims of the proposal are two-fold. First, the fabrication, characterization and optimization of nanoscale, electrochemical aptamer-based sensors will lead to high-performance sensors capable of specifically monitoring adenosine triphosphate (ATP) release from astrocytes. Second, sensors will be used to determine the dominant mechanism(s) of ATP release and whether ATP release and mechanisms are localized to specific regions of the astrocyte. The initial focus in this proposal will be the development of in vitro measurements with the nanosensors on cultured astrocytes. However, because the sensors developed function in complex biological media, they will, in the longer term, be applied to more physiological models (brain slices) and ultimately optimized for use in vivo to elucidate the role of ATP as a signaling molecule. The long-term goal of the research is to understand the molecular basis of glial cell communication in order to accelerate discoveries and reduce the burden of nervous system disorders. The proposed studies will yield a cutting edge tool that that is capable of transforming glial cell research by providing methodology to understand function in the central nervous system.

Public Health Relevance

The studies outlined in this proposal focus on the development of a nanoscale electrochemical sensor that will be used to determine the role of specific chemical messengers in the central nervous system. The enhanced characteristics of the sensor platform relative to current methodologies will provide a powerful new tool to study the molecular basis of cellular communication. Additionally, the sensing platform developed will, in the intermediate and long term, be applicable to in vivo models to accelerate discoveries and reduce the burden of nervous system disorders.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21MH101692-01A1
Application #
8701840
Study Section
Special Emphasis Panel (BNVT)
Program Officer
Nadler, Laurie S
Project Start
2014-04-03
Project End
2016-03-31
Budget Start
2014-04-03
Budget End
2015-03-31
Support Year
1
Fiscal Year
2014
Total Cost
$188,113
Indirect Cost
$56,505
Name
University of Maryland Balt CO Campus
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
061364808
City
Baltimore
State
MD
Country
United States
Zip Code
21250