Behavioral and physiological responses to psychosocial stressors can predict the long-term health consequences of exposure to stress. These consequences include dramatically elevated risks for cardiometabolic diseases, addictions, and mental disorders. Little is known, however, about either the genetic variation underlying inter-individual variation in susceptibility to stress, or about the gene expression patterns that provide the presumed mechanism for such variation. We propose to create, using the Caribbean vervet monkey (Chlorocebus aethiops sabaeus), a translational non-human primate (NHP) model for stress responsivity studies, which will facilitate investigations of the multi-system effects of an acute psychosocial stressor on physiology, behavior and cognition. These investigations are not feasible in humans, and cannot be reliably modeled in rodents, which differ from humans too substantially in these systems. We will leverage the short-term quarantine procedures applied routinely to wild-trapped vervets on the island of St. Kitts to investigate multi-system measures hypothesized to be associated with stress-responsivity. Specifically, we will longitudinally monitor expression of genes and gene networks that are stress-regulated and that predict individual differences in behavioral and physiological sensitivity to stress. This proof of concept study will validate the proposed model by: 1) demonstrating the impact of the stressor on metabolic, inflammatory and behavioral responses;2) characterizing inter-individual variations in stress-related traits;3) showing mechanistic links between stress-induced changes in higher-order phenotypes and gene expression patterns. Development of this model will, in the short-term provide insights into the molecular underpinnings of stress- related diseases, and, in the long-term, pave the way for large scale genetic and genomic investigations to identify the basis for individual differences in response to stress.

Public Health Relevance

This project will develop a translational animal model using genome-wide gene expression profiles to understand the health effects of psychosocial stress. By discovering the genes and gene networks that explain the links between stress and physiology, we will uncover mediators of stress effects in the functions of multiple organ systems, thus identifying new markers of health risks and targets for intervention. This study will demonstrate the feasibility of the proposed model for future large-scale studies aimed at discovering genetic variants contributing to vulnerability to stress.

Agency
National Institute of Health (NIH)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21OD017959-01
Application #
8624375
Study Section
Biobehavioral Regulation, Learning and Ethology Study Section (BRLE)
Program Officer
Moro, Manuel H
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of California Los Angeles
Department
None
Type
Overall Medical
DUNS #
City
Los Angeles
State
CA
Country
United States
Zip Code
90095