The purpose of this proposal is to advance use of the zebrafish as an applied tool for helping to diagnose and develop treatments for human disease. This proposal aims to develop and test a new method for using the zebrafish to measure the relative activity and potential pathogenicity of allelic forms of human genes. Methods will be developed that allow the removal of specific zebrafish genes on demand and the expression of human genes in the zebrafish. As a test case, this proposal uses the new methods to determine if the zebrafish can be used to distinguish between normal and disease-causing forms of the KCNH2 gene, a gene that when defective leads to Long QT Syndrome.

Public Health Relevance

This proposal aims to establish a new method for using the zebrafish to measure the relative activity and potential pathogenicity of allelic forms of human genes. Methods will be developed that allow the removal of specific zebrafish genes on demand and the expression of human genes in the zebrafish. As a test case, this proposal uses the new methods to determine if the zebrafish can be used to distinguish between normal and disease-causing forms of the KCNH2 gene, a gene that when defective leads to Long QT Syndrome.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21OD018323-01
Application #
8684468
Study Section
Development - 2 Study Section (DEV2)
Program Officer
Chang, Michael
Project Start
2014-04-01
Project End
2016-03-31
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
1
Fiscal Year
2014
Total Cost
$223,500
Indirect Cost
$73,500
Name
University of Utah
Department
Genetics
Type
Schools of Medicine
DUNS #
009095365
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112