The objective of this research is to determine the effects of dietary zinc deficiency on lymphocyte function during the different stages of development. The inbred mouse will continue to serve as a model system for study. A fairly complete profile of the effect of zinc deficiency on immunity in the young adult mouse has been developed by our laboratory. In the current application, we propose to study the effects of zinc deficiency on lymphopoiesis. To accomplish this, bone marrow stem cells prepared from zinc-deficient mice will be examined for their ability to migrate to, colonize, and proliferate in the lymphatic organs. In addition, the effect of periods of suboptimal zinc on development of immunity during the fetal-neonatal period will be investigated. Preliminary evidence suggests that zinc deficiency during ontogeny does alter lymphocyte development. In addition, pilot studies suggest that immune damage incurred during fetal development may not be fully repairable upon nutritional repletion. Thus determining the immune repair capacity of previously zinc-deficient fetal-neonatal mice will represent an additional area of investigation.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Unknown (R22)
Project #
5R22HD010586-09
Application #
3445104
Study Section
Immunological Sciences Study Section (IMS)
Project Start
1976-12-01
Project End
1986-06-30
Budget Start
1984-12-01
Budget End
1986-06-30
Support Year
9
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Michigan State University
Department
Type
Schools of Osteopathy
DUNS #
193247145
City
East Lansing
State
MI
Country
United States
Zip Code
48824
King, L E; Osati-Ashtiani, F; Fraker, P J (1995) Depletion of cells of the B lineage in the bone marrow of zinc-deficient mice. Immunology 85:69-73
Telford, W G; Fraker, P J (1995) Preferential induction of apoptosis in mouse CD4+CD8+ alpha beta TCRloCD3 epsilon lo thymocytes by zinc. J Cell Physiol 164:259-70
Fraker, P J; Osati-Ashtiani, F; Wagner, M A et al. (1995) Possible roles for glucocorticoids and apoptosis in the suppression of lymphopoiesis during zinc deficiency: a review. J Am Coll Nutr 14:11-7
Fraker, P J; King, L E; Lill-Elghanian, D et al. (1995) Quantification of apoptotic events in pure and heterogeneous populations of cells using the flow cytometer. Methods Cell Biol 46:57-76
Telford, W G; King, L E; Fraker, P J (1994) Rapid quantitation of apoptosis in pure and heterogeneous cell populations using flow cytometry. J Immunol Methods 172:1-16
Garvy, B A; Telford, W G; King, L E et al. (1993) Glucocorticoids and irradiation-induced apoptosis in normal murine bone marrow B-lineage lymphocytes as determined by flow cytometry. Immunology 79:270-7
Cook-Mills, J M; Fraker, P J (1993) Functional capacity of the residual lymphocytes from zinc-deficient adult mice. Br J Nutr 69:835-48
Garvy, B A; King, L E; Telford, W G et al. (1993) Chronic elevation of plasma corticosterone causes reductions in the number of cycling cells of the B lineage in murine bone marrow and induces apoptosis. Immunology 80:587-92
King, L E; Fraker, P J (1991) Flow cytometric analysis of the phenotypic distribution of splenic lymphocytes in zinc-deficient adult mice. J Nutr 121:1433-8
Garvy, B A; Fraker, P J (1991) Suppression of the antigenic response of murine bone marrow B cells by physiological concentrations of glucocorticoids. Immunology 74:519-23

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