Many diseases that affect the human brain cannot be studied using animal models. In recent years new techniques have been developed in histology and molecular biology that can be applied to human post-mortem (autopsy) brain tissue. These studies have enabled neuroscientists to make significant advances in understanding the causes and mechanisms of many diseases that damage the brain. Further progress in these types of studies is dependent upon the continued supply of autopsy brain tissue. A human brain tissue resource center is being developed in the Neuropathology Unit at the University of Sydney.
The aim of this important facility is to provide fresh-frozen and formalin fixed brain tissue to neuroscientists with an interest in alcohol- related disorders. Studies on dependence and tolerance, and mechanisms underlying structural changes in the brain are all feasible. New molecular biological techniques will also facilitate a range of genetic studies. Per capita consumption of alcohol in Australia is very high and alcohol-related brain damage is common. The principal investigator has been studying the long-term pathological effects of alcohol on the brain for 15 years and has collaborated in neuropharmacological, neurochemical and genetic studies. There are almost 3000 hospital and forensic autopsies per year in Sydney. One of the most important aspects of """"""""brain banking"""""""" is the accuracy and reliability of clinical and pathological diagnoses. Clinical diagnoses are confirmed by interviews with local doctors, study of past hospital medical records, questionnaires to next-of-kin and by analysis of pathology, radiology and neuropsychology reports. Autopsy and toxicology findings are also important in deciding whether or not a case fulfills the strict criteria for banking. Cases are selected from Hospital and Forensic autopsies by the Principal Investigator and are transferred to the Neuropathology Unit in preparation for diagnostic neuropathology and banking. Half the brain is fixed in formalin and half is dessected and frozen at minus 80 degrees C. The formalin-fixed hemisphere is embedded in agarose and sliced into approximately sixty, 3mm thick, slices that are carefully examined and photographed. Photographs are digital and are burnt onto a CD for permanent storage and easy retrieval. They are used for measuring areas and volumes of regions of interest. Tissue blocks are taken from cerebral cortex, basal ganglia, thalamus and hypothalamus, cerebellum and brain-stem and are processed by embedding in wax. Thin (10mu) sections are cut, stained and mounted on glass slides for microscopic examination to identify abnormalities and exclude diseases such as Alzheimer's disease. Cases are coded for confidentiality and all clinical and pathological information is entered onto a data base. Expressions of interest will be sought from research groups, interested in obtaining material from the tissue resource center. Applications will be processed by a scientific review committee. Ethics approval from the parent Institution is mandatory. All collaborating groups must provide annual research reports and list publications arising from the work. A """"""""bank"""""""" of this type is necessarily a long-term project and the maximum benefits will be seen as case numbers increase and subgroups of cases (eg males versus females) can be selectively studied.

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Resource-Related Research Projects (R24)
Project #
5R24AA012725-02
Application #
6371651
Study Section
Health Services Research Review Subcommittee (AA)
Program Officer
Sorensen, Roger
Project Start
2000-09-01
Project End
2003-08-31
Budget Start
2001-09-01
Budget End
2002-08-31
Support Year
2
Fiscal Year
2001
Total Cost
$204,714
Indirect Cost
Name
University of Sydney
Department
Type
DUNS #
752389338
City
Sydney
State
Country
Australia
Zip Code
2006
Youssef, Priscilla; Chami, Belal; Lim, Julia et al. (2018) Evidence supporting oxidative stress in a moderately affected area of the brain in Alzheimer's disease. Sci Rep 8:11553
Trist, Benjamin G; Davies, Katherine M; Cottam, Veronica et al. (2017) Amyotrophic lateral sclerosis-like superoxide dismutase 1 proteinopathy is associated with neuronal loss in Parkinson's disease brain. Acta Neuropathol 134:113-127
Dzamko, Nicolas; Gysbers, Amanda; Perera, Gayathri et al. (2017) Toll-like receptor 2 is increased in neurons in Parkinson's disease brain and may contribute to alpha-synuclein pathology. Acta Neuropathol 133:303-319
Genoud, Sian; Roberts, Blaine R; Gunn, Adam P et al. (2017) Subcellular compartmentalisation of copper, iron, manganese, and zinc in the Parkinson's disease brain. Metallomics 9:1447-1455
Glass, L J; Sinclair, D; Boerrigter, D et al. (2017) Brain antibodies in the cortex and blood of people with schizophrenia and controls. Transl Psychiatry 7:e1192
Coleman Jr, Leon G; Zou, Jian; Crews, Fulton T (2017) Microglial-derived miRNA let-7 and HMGB1 contribute to ethanol-induced neurotoxicity via TLR7. J Neuroinflammation 14:22
Andrews, Jessica L; Goodfellow, Frederic J; Matosin, Natalie et al. (2017) Alterations of ubiquitin related proteins in the pathology and development of schizophrenia: Evidence from human and animal studies. J Psychiatr Res 90:31-39
Chen, Bei Jun; Mills, James D; Takenaka, Konii et al. (2016) Characterization of circular RNAs landscape in multiple system atrophy brain. J Neurochem 139:485-496
Allen, Katherine M; Fung, Samantha J; Weickert, Cynthia Shannon (2016) Cell proliferation is reduced in the hippocampus in schizophrenia. Aust N Z J Psychiatry 50:473-80
Weissleder, Christin; Fung, Samantha J; Wong, Matthew W et al. (2016) Decline in Proliferation and Immature Neuron Markers in the Human Subependymal Zone during Aging: Relationship to EGF- and FGF-Related Transcripts. Front Aging Neurosci 8:274

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