The preparation and availability of glycan microarrays was developed by the NIGMS/NIH through a Glue-Grant-supported Consortium for Functional Glycomics (CFG). This resource has revolutionized the glycomics field through making available a rich assortment of over 500 immobilized glycans for general screening for interactions with proteins that recognize glycan determinants (glycan-binding proteins or GBPs). This is an application for continued support of this key """"""""legacy"""""""" resource due to the expiration of funding for the CFG in July 2011, following successful work for 10 years under the Glue Grant mechanism. Here we propose to continue this resource as the """"""""Protein-Glycan Interaction Resource of the CFG"""""""", in which two of the key cores of the CFG, Core D and Core H, will be combined to make available glycan microarrays for wide use by biomedical research community. As currently, investigators interested in using this resource will submit their request to the CFG Website that will be maintained through other legacy funding to the Bioinformatics Core B of the CFG. As currently, a Steering Committee of eminent scientists in the field will periodically review and approve/deny resources for investigators based on the scientific importance and resources available. Following approval, investigators will send samples to Emory University School of Medicine where the Protein-Glycan Interaction Resource will be housed in space currently used by Core H of the CFG and coordinated by Dr. Richard D. Cummings (PD/PI). The glycan microarrays used by the Protein-Glycan Interaction Resource will be provided by the Glycan Array Production Resource coordinated by Dr. James Paulson (PD/PI) at The Scripps Research Institute, and will initially be Version 5.0, but can be expanded as glycans may be contributed to the program by investigators in the field. Glycan microarray analyses will be conducted at Emory and completed results made available to the investigators and to the legacy bioinformatics of the CFG for public posting and curation. This program will ensure the highest level of integrity and systematic excellence in the preparation, utilization, and management of glycan microarray data, and make available to the broad research community this valuable resource.

Public Health Relevance

Glycan microarrays are used by hundreds of different investigators that have accessed them through the NIGMS/NIH Glue-Grant-supported Consortium for Functional Glycomics (CFG). This is a request for legacy funding to continue making available the glycan microarrays and associated databases and bioinformatics capabilities for the broad research community. This resource is unique and not duplicated elsewhere.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Resource-Related Research Projects (R24)
Project #
5R24GM098791-02
Application #
8332265
Study Section
Special Emphasis Panel (ZGM1-PPBC-4 (LR))
Program Officer
Marino, Pamela
Project Start
2011-09-20
Project End
2014-08-31
Budget Start
2012-09-01
Budget End
2013-08-31
Support Year
2
Fiscal Year
2012
Total Cost
$883,158
Indirect Cost
$216,337
Name
Emory University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
066469933
City
Atlanta
State
GA
Country
United States
Zip Code
30322
Bishnoi, Ritika; Mahajan, Sonal; Ramya, T N C (2018) An F-type lectin domain directs the activity of Streptosporangium roseum alpha-l-fucosidase. Glycobiology 28:860-875
Sadana, Pooja; Geyer, Rebecca; Pezoldt, Joern et al. (2018) The invasin D protein from Yersinia pseudotuberculosis selectively binds the Fab region of host antibodies and affects colonization of the intestine. J Biol Chem 293:8672-8690
Sharma, Mamta; Hegde, Prajna; Hiremath, Kavita et al. (2018) Purification, characterization and fine sugar specificity of a N-Acetylgalactosamine specific lectin from Adenia hondala. Glycoconj J 35:511-523
Mahajan, Sonal; Ramya, T N C (2018) Nature-inspired engineering of an F-type lectin for increased binding strength. Glycobiology 28:933-948
Hu, Liya; Sankaran, Banumathi; Laucirica, Daniel R et al. (2018) Glycan recognition in globally dominant human rotaviruses. Nat Commun 9:2631
Sun, Xiaoman; Li, Dandi; Qi, Jianxun et al. (2018) Glycan Binding Specificity and Mechanism of Human and Porcine P[6]/P[19] Rotavirus VP8*s. J Virol 92:
Sun, Xiaoman; Wang, Lihong; Qi, Jianxun et al. (2018) Human Group C Rotavirus VP8*s Recognize Type A Histo-Blood Group Antigens as Ligands. J Virol 92:
Sun, Hailiang; Kaplan, Bryan S; Guan, Minhui et al. (2017) Pathogenicity and transmission of a swine influenza A(H6N6) virus. Emerg Microbes Infect 6:e17
Tanaka, Koji; Caaveiro, Jose M M; Morante, Koldo et al. (2017) Haemolytic actinoporins interact with carbohydrates using their lipid-binding module. Philos Trans R Soc Lond B Biol Sci 372:
Tati, Swetha; Fisk, John C; Abdullah, Julia et al. (2017) Humanization of JAA-F11, a Highly Specific Anti-Thomsen-Friedenreich Pancarcinoma Antibody and InVitro Efficacy Analysis. Neoplasia 19:716-733

Showing the most recent 10 out of 87 publications