The nematode, Caenorhabditis elegans, has been identified as a """"""""high connectivity"""""""" animal for cellular development. This very small, simple animal has been widely adopted for experimental study due to its excellent genetics and accessible cell biology. The complete genome of the worm has been published, and most of the adult anatomy has been reconstructed from electron micrographs of serial thin sections. However, EM techniques are not widely utilized by the C. elegans community, and most previous EM data are not easily accessed. The Center for C. elegans Anatomy concentrates on extending the EM methods on C. elegans, particularly to 1) study cellular changes during normal development and in mutant animals, and 2) conduct EM-immunocytochemical and histochemical studies to localize antigens or molecules at the ultrastructurallevel. Such studies are being conducted in close collaboration with NIH- and NSF-funded laboratories, in fulfillment of their existing experimental aims. In addition, the Center is 3)publishingdetailed information regarding the anatomy of the nematode in the form of annotated TEM and light micrographs. Information is available on the Internet on our WormAtlas and Wormlmage websites, by ftp, on CDs and DVDs, and now in book form in the C. elegans Atlas. Where necessary, the Center is 4) testing new EM methods for C. elegans, and conducting new EM surveys of the wild type anatomy at key developmental stages to supplement the Atlas, and to fill gaps in our general knowledge. The Center also provides 5) training in TEM methods so that more C. elegans labs can conduct their own EM studies. This R24 application was first funded and the Center opened in Feb. 1998;we request five years of funding to continue building our capabilities to serve the scientificcommunity.

Public Health Relevance

Many disease-related gene sequences are known in the C. elegans genome;the TEM pathology of genetic mutations in nematode is often helpful in discovering the function of equivalent gene products in man. Studies in this laboratory have uncovered basic mechanisms related to development of the brain, and explored genes involved in several forms of cell death, including apoptosis, necrosis and autophagy.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Resource-Related Research Projects (R24)
Project #
8R24OD010943-15
Application #
8232063
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Program Officer
Chang, Michael
Project Start
1998-02-01
Project End
2014-02-28
Budget Start
2012-03-01
Budget End
2013-02-28
Support Year
15
Fiscal Year
2012
Total Cost
$727,779
Indirect Cost
$278,202
Name
Albert Einstein College of Medicine
Department
Neurosciences
Type
Schools of Medicine
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
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Nichols, Annika L A; Meelkop, Ellen; Linton, Casey et al. (2016) The Apoptotic Engulfment Machinery Regulates Axonal Degeneration in C. elegans Neurons. Cell Rep 14:1673-83
Morsci, Natalia S; Hall, David H; Driscoll, Monica et al. (2016) Age-Related Phasic Patterns of Mitochondrial Maintenance in Adult Caenorhabditis elegans Neurons. J Neurosci 36:1373-85
Johnsen, Holly L; Horvitz, H Robert (2016) Both the apoptotic suicide pathway and phagocytosis are required for a programmed cell death in Caenorhabditis elegans. BMC Biol 14:39
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Santella, Anthony; Kovacevic, Ismar; Herndon, Laura A et al. (2016) Digital development: a database of cell lineage differentiation in C. elegans with lineage phenotypes, cell-specific gene functions and a multiscale model. Nucleic Acids Res 44:D781-5
Dimitriadi, Maria; Derdowski, Aaron; Kalloo, Geetika et al. (2016) Decreased function of survival motor neuron protein impairs endocytic pathways. Proc Natl Acad Sci U S A 113:E4377-86
Stavoe, Andrea K H; Hill, Sarah E; Hall, David H et al. (2016) KIF1A/UNC-104 Transports ATG-9 to Regulate Neurodevelopment and Autophagy at Synapses. Dev Cell 38:171-85
Gleason, Adenrele M; Nguyen, Ken C Q; Hall, David H et al. (2016) Syndapin/SDPN-1 is required for endocytic recycling and endosomal actin association in the C. elegans intestine. Mol Biol Cell :
Maguire, Julie E; Silva, Malan; Nguyen, Ken C Q et al. (2015) Myristoylated CIL-7 regulates ciliary extracellular vesicle biogenesis. Mol Biol Cell 26:2823-32

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