The nematode, Caenorhabditis elegans, has been identified as a "high connectivity" animal for cellular development. This very small, simple animal has been widely adopted for experimental study due to its excellent genetics and accessible cell biology. The complete genome of the worm has been published, and most of the adult anatomy has been reconstructed from electron micrographs of serial thin sections. However, EM techniques are not widely utilized by the C. elegans community, and most previous EM data are not easily accessed. The Center for C. elegans Anatomy concentrates on extending the EM methods on C. elegans, particularly to 1) study cellular changes during normal development and in mutant animals, and 2) conduct EM-immunocytochemical and histochemical studies to localize antigens or molecules at the ultrastructurallevel. Such studies are being conducted in close collaboration with NIH- and NSF-funded laboratories, in fulfillment of their existing experimental aims. In addition, the Center is 3)publishingdetailed information regarding the anatomy of the nematode in the form of annotated TEM and light micrographs. Information is available on the Internet on our WormAtlas and Wormlmage websites, by ftp, on CDs and DVDs, and now in book form in the C. elegans Atlas. Where necessary, the Center is 4) testing new EM methods for C. elegans, and conducting new EM surveys of the wild type anatomy at key developmental stages to supplement the Atlas, and to fill gaps in our general knowledge. The Center also provides 5) training in TEM methods so that more C. elegans labs can conduct their own EM studies. This R24 application was first funded and the Center opened in Feb. 1998;we request five years of funding to continue building our capabilities to serve the scientificcommunity.

Public Health Relevance

Many disease-related gene sequences are known in the C. elegans genome;the TEM pathology of genetic mutations in nematode is often helpful in discovering the function of equivalent gene products in man. Studies in this laboratory have uncovered basic mechanisms related to development of the brain, and explored genes involved in several forms of cell death, including apoptosis, necrosis and autophagy.

National Institute of Health (NIH)
Office of The Director, National Institutes of Health (OD)
Resource-Related Research Projects (R24)
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National Center for Research Resources Initial Review Group (RIRG)
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Chang, Michael
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Albert Einstein College of Medicine
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George, Divya T; Behm, Carolyn A; Hall, David H et al. (2014) Shigella flexneri infection in Caenorhabditis elegans: cytopathological examination and identification of host responses. PLoS One 9:e106085
Possik, Elite; Jalali, Zahra; Nou√ęt, Yann et al. (2014) Folliculin regulates ampk-dependent autophagy and metabolic stress survival. PLoS Genet 10:e1004273
Wang, Juan; Silva, Malan; Haas, Leonard A et al. (2014) C. elegans ciliated sensory neurons release extracellular vesicles that function in animal communication. Curr Biol 24:519-25
Zhang, Jingyan; Li, Xia; Jevince, Angela R et al. (2013) Neuronal target identification requires AHA-1-mediated fine-tuning of Wnt signaling in C. elegans. PLoS Genet 9:e1003618