The last several years have witnessed an explosion in our understanding of vertebrate development, largely based on work from a few model genetic organisms. The embryonic zebrafish is one of these, and many research grants are funded by NIH and NIEHS to utilize this model. Studies of the embryology and genetics of zebrafish and the recent identification of 1,000's of genetic mutations have lead to this dramatic increase in the number of laboratories (now ca 450) using zebrafish to study the basic mechanisms of vertebrate developmental biology. Although zebrafish have become an extremely important research model, relatively little is known about the diseases that affect them when held in captivity. Indeed, severe health problems in zebrafish research colonies could seriously jeopardize many millions of dollars in research funding, and some research facilities have experienced devastating, acute mortalities in their zebrafish colonies. Moreover, persistent, but less severe, infections have plagued several facilities. As with other laboratory animals used in research, it is imperative to conduct studies with disease-free, healthy zebrafish. This is even more important now as several researchers are now using zebrafish as models in long-term studies on human pathogens and in toxicology. Unlike rodent models, there are no certified specific pathogen free (SPF) stocks of zebrafish, and we are just now developing sensitive diagnostic tests for the most serious pathogens. The major aim of the present study is to improve the health of zebrafish used in research facilities by elucidation of the pathogenesis and modes of transmission of the most important pathogens in this fish - e.g., Pseudoloma neurophilia, a microsporidium of the central nervous system that is very prevalent in zebrafish, and Mycobacterium spp, the cause offish tuberculosis. P. neurophilia is extremely prevalent in zebrafish facilities and evidence indicates that it is transmitted vertically. We will therefore development of a non-lethal diagnostic test for brood fish, and investigate methods to disinfect eggs. For mycobacteria, we will determine which strains or species are most virulent, as we see dramatic differences in mortalities between outbreaks. PCR tests will be made for the virulent strains. Results will be quickly distributed to the zebrafish community through ZFIN, peer reviewed publications, and our web based manual on zebrafish diseases

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Resource-Related Research Projects (R24)
Project #
8R24OD010998-10
Application #
8264971
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Program Officer
Chang, Michael
Project Start
2003-07-01
Project End
2013-07-18
Budget Start
2012-06-01
Budget End
2013-05-31
Support Year
10
Fiscal Year
2012
Total Cost
$211,791
Indirect Cost
$63,291
Name
Oregon State University
Department
Microbiology/Immun/Virology
Type
Schools of Earth Sciences/Natur
DUNS #
053599908
City
Corvallis
State
OR
Country
United States
Zip Code
97339
Martins, M L; Watral, V; Rodrigues-Soares, J P et al. (2016) A method for collecting eggs of Pseudocapillaria tomentosa (Nematoda: Capillariidae) from zebrafish Danio rerio and efficacy of heat and chlorine for killing the nematode's eggs. J Fish Dis :
Spagnoli, S; Sanders, J; Kent, M L (2016) The common neural parasite Pseudoloma neurophilia causes altered shoaling behaviour in adult laboratory zebrafish (Danio rerio) and its implications for neurobehavioural research. J Fish Dis :
Nogueira, Christiane Lourenço; Whipps, Christopher M; Matsumoto, Cristianne Kayoko et al. (2015) Mycobacterium saopaulense sp. nov., a rapidly growing mycobacterium closely related to members of the Mycobacterium chelonae--Mycobacterium abscessus group. Int J Syst Evol Microbiol 65:4403-9
Paquette, Colleen E; Kent, Michael L; Peterson, Tracy S et al. (2015) Immunohistochemical characterization of intestinal neoplasia in zebrafish Danio rerio indicates epithelial origin. Dis Aquat Organ 116:191-7
Spagnoli, Sean; Xue, Lan; Kent, Michael L (2015) The common neural parasite Pseudoloma neurophilia is associated with altered startle response habituation in adult zebrafish (Danio rerio): Implications for the zebrafish as a model organism. Behav Brain Res 291:351-60
Chang, Carolyn T; Whipps, Christopher M (2015) Activity of Antibiotics against Mycobacterium Species Commonly Found in Laboratory Zebrafish. J Aquat Anim Health 27:88-95
Spagnoli, Sean Thomas; Xue, Lan; Murray, Katrina N et al. (2015) Pseudoloma neurophilia: a retrospective and descriptive study of nervous system and muscle infections, with new implications for pathogenesis and behavioral phenotypes. Zebrafish 12:189-201
Whipps, Christopher M; Murray, Katrina N; Kent, Michael L (2015) Occurrence of a myxozoan parasite Myxidium streisingeri n. sp. in laboratory zebrafish Danio rerio. J Parasitol 101:86-90
Chang, Carolyn T; Colicino, Erica G; DiPaola, Elizabeth J et al. (2015) Evaluating the effectiveness of common disinfectants at preventing the propagation of Mycobacterium spp. isolated from zebrafish. Comp Biochem Physiol C Toxicol Pharmacol 178:45-50
West, Kylie; Miles, Rodney; Kent, Michael L et al. (2014) Unusual fluorescent granulomas and myonecrosis in Danio rerio infected by the microsporidian pathogen Pseudoloma neurophilia. Zebrafish 11:283-90

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