Novel scientific contributions that would come from the exciting exploration of aquatic experimental models that each have evolved new ways to survive within exceedingly varied and stressful physical and biological environments is our foremost contention for the sequencing and assembly of the genomes of the species listed in this proposal. Many long-standing aquatic research models have notable and noble places in the history of our understanding of human disease. In addition, distinct attributes of these models provided plasticity in experimental design and a breadth of discovery needed to light the fires of creative inquiry. Such has been the historical roles for non-mammalian and aquatic animal models. However, the power of genomics over the past decade has revolutionized our understanding of the molecular basis of human disease. Unfortunately, due to high costs associated with the development of genomic resources, this advance has been limited to only a handful of popular and established animal models. Naturally, concurrent with the initial rise of genomics capabilities for a few model organisms there came reduced interest in support for comparative biological scientific inquiry that employed species that did not possess such resources. Previously we had organized a meeting, entitled """"""""Aquatic Models for Human Disease"""""""", to gather a broad community of aquatic researchers seeking to gain input toward obtaining a list of species that reflect a broad diversity of species-specific biology. More importantly the focus of this discussion was to choose species that would provide a means to explore the biology that indirectly reflects a particular human disease. From this discussion and a genomics workshop held prior to the meeting, we have compiled a priority list of species with model organism proven capabilities (Table 1). Herein we describe our plans to generate genome resources for nine aquatic species that will empower scientists to test novel hypotheses for a wide array of human disease pathway origins. The great promise to forward our understanding of human disease using these proposed aquatic genomes is now warranted.
(provided by applicant): There is a real need to continue to develop and understand non-human models of human disease. The natural course of a disease in a human may take years to manifest symptoms;to overcome this problem, investigators have developed experimentally tractable models employing organisms that can mimic a disease-in a period of months to a few years and with genetic power. We have thus far not established the resources needed to take advantage of the aquatic species having unique attributes that mimic a wide array of human diseases.
|Schauer, Kevin L; LeMoine, Christophe M R; Pelin, Adrian et al. (2016) A proteinaceous organic matrix regulates carbonate mineral production in the marine teleost intestine. Sci Rep 6:34494|
|Gross, Joshua B; Stahl, Bethany A; Powers, Amanda K et al. (2016) Natural bone fragmentation in the blind cave-dwelling fish, Astyanax mexicanus: candidate gene identification through integrative comparative genomics. Evol Dev 18:7-18|
|Schartl, Manfred; Shen, Yingjia; Maurus, Katja et al. (2015) Whole Body Melanoma Transcriptome Response in Medaka. PLoS One 10:e0143057|
|McGaugh, Suzanne E; Gross, Joshua B; Aken, Bronwen et al. (2014) The cavefish genome reveals candidate genes for eye loss. Nat Commun 5:5307|
|Flicek, Paul; Amode, M Ridwan; Barrell, Daniel et al. (2014) Ensembl 2014. Nucleic Acids Res 42:D749-55|
|Kowalko, Johanna E; Rohner, Nicolas; Linden, Tess A et al. (2013) Convergence in feeding posture occurs through different genetic loci in independently evolved cave populations of Astyanax mexicanus. Proc Natl Acad Sci U S A 110:16933-8|
|Schartl, Manfred; Walter, Ronald B; Shen, Yingjia et al. (2013) The genome of the platyfish, Xiphophorus maculatus, provides insights into evolutionary adaptation and several complex traits. Nat Genet 45:567-72|