Glucose is the major precursor for lactose and lipid synthesis in the lactating mammary gland, and determines the volume and energy content of milk. The long-term objective is to understand, at the molecular and cellular levels, the mechanisms and regulation of glucose transport in milk synthesis and secretion.
Specific aims are: 1) to investigate the developmental regulation of glucose transport in the mammary gland; 2) to study the developmental and hormonal regulations of glucose transport in mammary epithelial cells in vitro; 3) to identify novel proteins involved in glucose transport in lactating mammary gland epithelia; and 4) to determine the role o glucose transport as a control mechanism and determinant of flux for the pathway of lactose synthesis. An improved understanding of the regulation of milk synthesis and secretion would form the basis for rational interventions t monitor and improve lactational performance, thereby prolonging the duration o breast feeding. Experiments will be performed in established cell lines, primary cells and intact and reconstituted mammary glands. Molecular cell biology techniques will be used to define developmental changes in the amount and subcellular localization of glucose transporter (GLUT1) and to identify other glucose transporters or protein affecting glucose transporter trafficking. These will be related to changes in glucose transport activity an lactose synthesis that are essential for milk production.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29HD034701-03
Application #
6182625
Study Section
Nutrition Study Section (NTN)
Program Officer
Grave, Gilman D
Project Start
1998-09-20
Project End
2003-07-31
Budget Start
2000-08-01
Budget End
2001-07-31
Support Year
3
Fiscal Year
2000
Total Cost
$99,960
Indirect Cost
Name
Baylor College of Medicine
Department
Pediatrics
Type
Schools of Medicine
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030
Haney, Peter M (2004) Glucose transport in lactation. Adv Exp Med Biol 554:253-61
Tsang, Stella W Y; Haney, Peter M (2003) Imaging plasma membrane proteins by confocal microscopy. Methods Mol Biol 227:351-8
Haney, P M (2001) Localization of the GLUT1 glucose transporter to brefeldin A-sensitive vesicles of differentiated CIT3 mouse mammary epithelial cells. Cell Biol Int 25:277-88
Nemeth, B A; Tsang, S W; Geske, R S et al. (2000) Golgi targeting of the GLUT1 glucose transporter in lactating mouse mammary gland. Pediatr Res 47:444-50