Our overarching hypothesis is that selective synaptic alterations in cortical glutamatergic systems occur with aging, compromising both synapse number and the molecular and morphologic components of plasticity required for learning and memory, thereby contributing to age-related cognitive impairment The current Specific Aims are: 1) To determine the age-related morphologic and molecular alterations in pyramidal cells and axospinous synapses in area 46 of dorsolateral prefrontal cortex (dlPFC) in rhesus monkey and their contribution to cognitive aging;2) To determine the age-related synaptic alterations in rhesus monkey hippocampus and the degree to which they predict decrements in the medial temporal lobe memory system; and 3) To identify changes in synaptic GluR profiles induced by LTP in young and aged rats, how they are altered in aging, and how such age-related alterations relate to memory performance mediated by hippocampus. We have made extensive progress in all three Specific Aims, and have revealed fundamental differences in the nature of synaptic aging between hippocampus and dIPFC as well as differences in the indices that are predictive of cognitive performance mediated by each region. In addition, we have revealed that the small, thin, highly plastic spines are vulnerable in dIPFC, whereas the mushroom spines are unaffected by aging. The MERIT extension will allow us to pursue these reflections of selective vulnerability in synaptic aging comprehensively, as well as pursue several new directions. First, with extended time we will be able to determine the molecular profile of vulnerable vs. resistant spine classes in dIPFC to provide a basis for therapeutic strategies. Second, we will have access to two additional cohorts of young and aged rhesus monkeys with structural and functional imaging built in to the design, and differential rearing conditions as a variable for one of the cohorts. Third, we have now developed the capacity to dramatically expand our neuronal reconstruction and spine analysis to additional neocortical regions, allowing for a comprehensive analysis of selective synaptic vulnerability across the neocortex. Fourth, we will expand our molecular targets to include links to both tau pathology and actin polymerization to provide a more mechanistic basis for alterations in neuronal/synaptic structure and plasticity. Fifth, we will expand our rat analyses to include PFC to test the hypothesis that the synaptic and circuit mechanisms in the PFC age similarly and can impair homologous cognitive functions across species. In all cases, we will expand our capacity to provide novel therapeutic targets based on molecular underpinnings of synaptic aging.

Public Health Relevance

This project is designed to reveal the synaptic basis for age-related cognitive decline. The emerging data will provide a framework for both behavioral and pharmaceutical therapies to protect against age-related cognitive decline. This is important for non-Alzheimer's Disease cognitive decline as well as Alzheimer's Disease since the synaptic alterations that we identify very likely occur in the human brain as well, leaving neurons vulnerable to the degenerative cascade embodied by Alzheimer's Disease.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37AG006647-25
Application #
8442264
Study Section
Special Emphasis Panel (NSS)
Program Officer
Wagster, Molly V
Project Start
1989-08-01
Project End
2017-03-31
Budget Start
2013-05-15
Budget End
2014-03-31
Support Year
25
Fiscal Year
2013
Total Cost
$481,704
Indirect Cost
$197,513
Name
Icahn School of Medicine at Mount Sinai
Department
Neurosciences
Type
Schools of Medicine
DUNS #
078861598
City
New York
State
NY
Country
United States
Zip Code
10029
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Dumitriu, Dani; Hao, Jiandong; Hara, Yuko et al. (2010) Selective changes in thin spine density and morphology in monkey prefrontal cortex correlate with aging-related cognitive impairment. J Neurosci 30:7507-15
Park, C S; Elgersma, Y; Grant, S G N et al. (2008) alpha-Isoform of calcium-calmodulin-dependent protein kinase II and postsynaptic density protein 95 differentially regulate synaptic expression of NR2A- and NR2B-containing N-methyl-d-aspartate receptors in hippocampus. Neuroscience 151:43-55

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