Lyme disease involves multiple organs, induding the skin, heart, joints and nervous system. Following a tick bite, 6. burgdorieri are deposited in the skin of the mammalian host. Spirochetes reside in the dermis for about one week and then spread to many tissues throughout the body. The spirochetes colonize the joints, causing arthritis in botii mice and humans. The goal of this proposal is to characterize B. /wnawtoffjerf genes important in spirochete dissemination from the skin and colonization of the iolnts. The Identified gene products will then be targeted to interfere with specific phases ofthe spirochete life cvcle. These studies will use the murine model of tick-bome fi. bu/gdbfferr infedion, which partially mimics human disease, and specimens from patients with welldocumented Lyme disease. These data will lead to a greater understanding of how fi. burgdorferi gene expression contrit>utes to spirochete infectivity, and suggest new strategies for the prevention and treatment of Lyme disease.
This project will lead to a greater understanding of the pathogenesis of, and Immunity against, Lyme disease. This infomnation will be useful for the development of new vaccines and therapeutics for this Important medical illness.
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|Krause, Peter J; Narasimhan, Sukanya; Wormser, Gary P et al. (2013) Human Borrelia miyamotoi infection in the United States. N Engl J Med 368:291-3|
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|Ullmann, A J; Dolan, M C; Sackal, C A et al. (2013) Immunization with adenoviral-vectored tick salivary gland proteins (SALPs) in a murine model of Lyme borreliosis. Ticks Tick Borne Dis 4:160-3|
|Magnarelli, Louis A; Williams, Scott C; Norris, Steven J et al. (2013) Serum antibodies to Borrelia burgdorferi, Anaplasma phagocytophilum, and Babesia microti in recaptured white-footed mice. J Wildl Dis 49:294-302|
|Narasimhan, Sukanya; Perez, Oriana; Mootien, Sara et al. (2013) Characterization of Ixophilin, a thrombin inhibitor from the gut of Ixodes scapularis. PLoS One 8:e68012|
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