Lyme disease involves multiple organs, induding the skin, heart, joints and nervous system. Following a tick bite, 6. burgdorieri are deposited in the skin of the mammalian host. Spirochetes reside in the dermis for about one week and then spread to many tissues throughout the body. The spirochetes colonize the joints, causing arthritis in botii mice and humans. The goal of this proposal is to characterize B. /wnawtoffjerf genes important in spirochete dissemination from the skin and colonization of the iolnts. The Identified gene products will then be targeted to interfere with specific phases ofthe spirochete life cvcle. These studies will use the murine model of tick-bome fi. bu/gdbfferr infedion, which partially mimics human disease, and specimens from patients with welldocumented Lyme disease. These data will lead to a greater understanding of how fi. burgdorferi gene expression contrit>utes to spirochete infectivity, and suggest new strategies for the prevention and treatment of Lyme disease.

Public Health Relevance

This project will lead to a greater understanding of the pathogenesis of, and Immunity against, Lyme disease. This infomnation will be useful for the development of new vaccines and therapeutics for this Important medical illness.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37AI049200-12
Application #
8236902
Study Section
Special Emphasis Panel (NSS)
Program Officer
Breen, Joseph J
Project Start
2001-05-01
Project End
2016-03-31
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
12
Fiscal Year
2012
Total Cost
$414,688
Indirect Cost
$164,688
Name
Yale University
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
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